CPLM-020356

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-020356

UniProt Accession

PDIP3_HUMAN; Q9BY77; A8K6F8; A8K6V9; Q009A7; Q5H972; Q6PGN6; Q7Z6Z0; Q9NSP5; Q9NSP6

Genbank Protein ID

AY422990; AB055760; AL160111; AL160112; CR456456; AK291623; AK291774; Z93241; Z93241; BC049840; BC056912; BC095411; DQ887818

Genbank Nucleotide ID

AAQ94604.1; BAB33368.2; CAB77058.1; CAB77059.1; CAG30342.1; BAF84312.1; BAF84463.1; CAI19835.1; CAQ08413.1; AAH49840.1; AAH56912.2; AAH95411.1; ABI74675.1

Protein Name

Polymerase delta-interacting protein 3

Protein Synonyms/Alias

46 kDa DNA polymerase delta interaction protein; p46; S6K1 Aly/REF-like target; SKAR

Gene Name

POLDIP3

Gene Synonyms/Alias

KIAA1649; PDIP46

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
147	TPALKLTKTIQNLYD	ubiquitination	[1]
171	SVPTKQMKFAASGGF	ubiquitination	[1]

Reference

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.

Sequence Annotation

DOMAIN 280 351 RRM.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 5 5 Phosphoserine.
MOD_RES 127 127 Phosphoserine.
MOD_RES 140 140 Phosphothreonine.
MOD_RES 275 275 Phosphoserine.
MOD_RES 383 383 Phosphoserine; by RPS6KB1.
MOD_RES 385 385 Phosphoserine; by RPS6KB1.

Keyword

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Translation regulation.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

421 AA

Protein Sequence

MADISLDELI RKRGAAAKGR LNARPGVGGV RSRVGIQQGL LSQSTRTATF QQRFDARQKI 60
GLSDARLKLG VKDAREKLLQ KDARFRIKGK VQDAREMLNS RKQQTTVPQK PRQVADAREK 120
ISLKRSSPAA FINPPIGTVT PALKLTKTIQ NLYDLDEDDD GIASVPTKQM KFAASGGFLH 180
HMAGLSSSKL SMSKALPLTK VVQNDAYTAP ALPSSIRTKA LTNMSRTLVN KEEPPKELPA 240
AEPVLSPLEG TKMTVNNLHP RVTEEDIVEL FCVCGALKRA RLVHPGVAEV VFVKKDDAIT 300
AYKKYNNRCL DGQPMKCNLH MNGNVITSDQ PILLRLSDSP SMKKESELPR RVNSASSSNP 360
PAEVDPDTIL KALFKSSGAS VTTQPTEFKI KL 392

Gene Ontology

GO:0005737; C:cytoplasm; TAS:UniProtKB.
GO:0016607; C:nuclear speck; IDA:UniProtKB.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0045727; P:positive regulation of translation; IMP:UniProtKB.

Interpro

IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.

Pfam

PF00076; RRM_1

SMART

SM00360; RRM

PROSITE

PS50102; RRM

PRINTS