CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023960
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin-4 
Protein Synonyms/Alias
 Myosin heavy chain 2b; MyHC-2b; Myosin heavy chain 4; Myosin heavy chain IIb; MyHC-IIb; Myosin heavy chain, skeletal muscle, fetal 
Gene Name
 MYH4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
1813QLALKGGKKQIQKLEubiquitination[1]
Reference
 [1] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265] 
Functional Description
 Muscle contraction. 
Sequence Annotation
 DOMAIN 1 784 Myosin head-like.
 DOMAIN 785 814 IQ.
 NP_BIND 179 186 ATP (Potential).
 REGION 659 681 Actin-binding (By similarity).
 REGION 761 775 Actin-binding (By similarity).
 MOD_RES 130 130 N6,N6,N6-trimethyllysine (Potential).
 MOD_RES 952 952 Phosphoserine (By similarity).
 MOD_RES 1203 1203 Phosphoserine (By similarity).
 MOD_RES 1928 1928 Phosphoserine (By similarity).
 MOD_RES 1933 1933 Phosphothreonine (By similarity).
 CROSSLNK 1813 1813 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Actin-binding; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Cytoplasm; Isopeptide bond; Methylation; Motor protein; Muscle protein; Myosin; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Thick filament; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1939 AA 
Protein Sequence
MSSDSEMAIF GEAAPFLRKS EKERIEAQNK PFDAKTSVFV VDPKESYVKA IVQSREGGKV 60
TAKTEAGATV TVKEDQVFSM NPPKYDKIED MAMMTHLHEP AVLYNLKERY AAWMIYTYSG 120
LFCVTVNPYK WLPVYNPEVV TAYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 180
SGAGKTVNTK RVIQYFATIA VTGEKKKEEP ASGKMQGTLE DQIISANPLL EAFGNAKTVR 240
NDNSSRFGKF IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QILSNKKPEL 300
IEMLLITTNP YDFAFVSQGE ITVPSIDDQE ELMATDSAVD ILGFTADEKV AIYKLTGAVM 360
HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK SLCYPRVKVG NEFVTKGQTV 420
QQVYNAVGAL AKAIYEKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC 480
INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE 540
CMFPKATDTS FKNKLYEQHL GKSNNFQKPK PAKGKPEAHF SLVHYAGTVD YNIAGWLDKN 600
KDPLNETVVG LYQKSAMKTL AFLFSGAQTA EAEGGGGKKG GKKKGSSFQT VSALFRENLN 660
KLMTNLRSTH PHFVRCIIPN ETKTPGAMEH ELVLHQLRCN GVLEGIRICR KGFPSRILYA 720
DFKQRYKVLN ASAIPEGQFI DSKKASEKLL GSIEIDHTQY KFGHTKVFFK AGLLGTLEEM 780
RDEKLAQLIT RTQAICRGFL MRVEFRKMME RRESIFCIQY NIRAFMNVKH WPWMKLYFKI 840
KPLLKSAETE KEMANMKEEF EKTKEELAKT EAKRKELEEK MVTLMQEKND LQLQVQAEAD 900
ALADAEERCD QLIKTKIQLE AKIKEVTERA EDEEEINAEL TAKKRKLEDE CSELKKDIDD 960
LELTLAKVEK EKHATENKVK NLTEEMAGLD ETIAKLTKEK KALQEAHQQT LDDLQMEEDK 1020
VNTLTKAKTK LEQQVDDLEG SLEQEKKLCM DLERAKRKLE GDLKLAQEST MDTENDKQQL 1080
NEKLKKKEFE MSNLQGKIED EQALAIQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR 1140
SDLSRELEEI SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEESTLQHE ATAAALRKKH 1200
ADSVAELGEQ IDSLQRVKQK LEKEKSELKM EINDLASNME TVSKAKANFE KMCRTLEDQL 1260
SEIKTKEEEQ QRLINELSAQ KARLHTESGE FSRQLDEKDA MVSQLSRGKQ AFTQQIEELK 1320
RQLEEETKAK STLAHALQSA RHDCDLLREQ YEEEQEAKAE LQRGMSKANS EVAQWRTKYE 1380
TDAIQRTEEL EEAKKKLAQR LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN 1440
AACIALDKKQ RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDHLE 1500
TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKVKKQLDHE KSELQTSLEE AEASLEHEEG 1560
KILRIQLELN QVKSEIDRKI AEKDEELDQL KRNHLRVVES MQSTLDAEIR SRNDALRIKK 1620
KMEGDLNEME IQLNHANRQA AEALRNLRNT QGILKDTQLH LDDAIRGQDD LKEQLAMVER 1680
RANLMQAEVE ELRASLERTE RGRKMAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ 1740
IQGEMEDIVQ EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQLR 1800
LDEAEQLALK GGKKQIQKLE ARVRELESEV ESEQKHNVEA VKGLRKHERR VKELTYQTEE 1860
DRKNILRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF RKLQHELEEA KERADIAESQ 1920
VNKLRVKSRE VHTKVISEE 1939 
Gene Ontology
 GO:0005925; C:focal adhesion; IDA:HPA.
 GO:0005859; C:muscle myosin complex; NAS:BHF-UCL.
 GO:0032982; C:myosin filament; IEA:UniProtKB-KW.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0030017; C:sarcomere; NAS:BHF-UCL.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000146; F:microfilament motor activity; NAS:UniProtKB.
 GO:0008307; F:structural constituent of muscle; NAS:BHF-UCL.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0030049; P:muscle filament sliding; NAS:BHF-UCL.
 GO:0014823; P:response to activity; IEA:Compara. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027401; Myosin-like_IQ_dom.
 IPR015650; Myosin_1/23/4/7/8/13/15.
 IPR001609; Myosin_head_motor_dom.
 IPR004009; Myosin_N.
 IPR002928; Myosin_tail.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00063; Myosin_head
 PF02736; Myosin_N
 PF01576; Myosin_tail_1 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.