CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007694
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar transporter chaperone 2 
Protein Synonyms/Alias
 Phosphate metabolism protein 1 
Gene Name
 VTC2 
Gene Synonyms/Alias
 PHM1; YFL004W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
315WTGQLSDKPDIFLEKacetylation[1]
416FQIPGDDKIRVTIDSacetylation[1]
459ANVANPLKFLRGGEYacetylation[1]
468LRGGEYAKFPYSVMEacetylation[1]
586MRRLSNLKEPQHQAAubiquitination[2]
627TEQEPHSKRSKKVRRubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. 
Sequence Annotation
 DOMAIN 1 146 SPX.
 MOD_RES 182 182 Phosphoserine.
 MOD_RES 187 187 Phosphoserine.
 MOD_RES 196 196 Phosphoserine.
 MOD_RES 264 264 Phosphoserine.
 MOD_RES 583 583 Phosphoserine.
 MOD_RES 615 615 Phosphoserine.
 MOD_RES 616 616 Phosphoserine.
 MOD_RES 620 620 Phosphothreonine.
 MOD_RES 626 626 Phosphoserine.
 MOD_RES 657 657 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 828 AA 
Protein Sequence
MLFGVKLANE VYPPWKGSYI NYEGLKKFLK EDSVKDGSND KKARWDDSDE SKFVEELDKE 60
LEKVYGFQLK KYNNLMERLS HLEKQTDTEA AIKALDADAF QRVLEELLSE STELDNFKRL 120
NFTGFAKIVK KHDKLYPKYP SVKSLLEVRL KELPSHSEEY SPLLYRISFL YNILRSNFNT 180
ASEPLASASK FSSIVSNDID MNFRSFKFWV HNDNLMEVKT RILRHLPVLV YANVPSENDD 240
LVNRFESDIS NNDEIVGSSS STSSVEHGLG ARSFDPLINT LYFDNEHFEL YNDKLLKLNS 300
APTLRLRWTG QLSDKPDIFL EKKTLIEDEA TGKSEFDLTK LQLKQKFING FIFEGDKKFK 360
EQTLKKLKES GTAGRDLERL EEDFSEIQNF IIKNELQPVF RTVYTRTAFQ IPGDDKIRVT 420
IDSNIVFIKE DSFDRERPIR DPNTWHRTDI DANVANPLKF LRGGEYAKFP YSVMEIKVKS 480
SLDSSMSASS MISNVKLPKK HGQWLNDLTN SHLVKEIPKF SIFVQGVASL YGDDEKLDIL 540
PFWLPDLETD IRQDPKQAYE EEKKKLLKQK EIQKKIDGMR RLSNLKEPQH QAAVPVSQEE 600
NERITSQGDL EADGSSDEET EQEPHSKRSK KVRRRKPKAT FLRILAGRDP KLMGVDSEEE 660
EIELPPGVKK PLNLLKNAGP VNVEAKVWLA NERTFNRWLS VTSLLSVLTF SIYNSVKKAE 720
YPTLANYMAY VYFGLTIFCA LWSYSIYMKR VDIIQQRSGQ HLDAPLGPVL VSIVLFVTLV 780
VNFVMAFRNA AKSRQELQIQ NLEVPERIPE VLRPLQNYLF KLMGPSSD 828 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0033254; C:vacuolar transporter chaperone complex; IPI:SGD.
 GO:0016237; P:microautophagy; IMP:SGD.
 GO:0006797; P:polyphosphate metabolic process; IMP:SGD.
 GO:0008104; P:protein localization; IMP:SGD.
 GO:0007034; P:vacuolar transport; IDA:SGD.
 GO:0042144; P:vacuole fusion, non-autophagic; IPI:SGD. 
Interpro
 IPR003807; DUF202.
 IPR004331; SPX_N.
 IPR018966; VTC_domain. 
Pfam
 PF02656; DUF202
 PF03105; SPX
 PF09359; VTC 
SMART
  
PROSITE
 PS51382; SPX 
PRINTS