UniProt Accession

 IF2A_MOUSE; Q6ZWX6; Q3TIQ0 

Genbank Protein ID

 AK010592; AK143499; AK167763; BC005463; BC016448; BC016497 

Genbank Nucleotide ID

 BAB27049.1; BAE25400.1; BAE39796.1; AAH05463.1; AAH16448.1; AAH16497.1 

Protein Name

 Eukaryotic translation initiation factor 2 subunit 1 

Protein Synonyms/Alias

 Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha 

Gene Name

 Eif2s1 

Gene Synonyms/Alias

 Eif2a 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
101	EAIKCEDKFTKSKTV	ubiquitination	[1]
106	EDKFTKSKTVYSILR	acetylation	[2]
106	EDKFTKSKTVYSILR	ubiquitination	[1]
141	TAWVFDDKYKRPGYG	acetylation	[2]
141	TAWVFDDKYKRPGYG	ubiquitination	[1]
259	NQAMAVIKEKIEEKR	acetylation	[2]
259	NQAMAVIKEKIEEKR	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity). 

Sequence Annotation

 DOMAIN 17 88 S1 motif.
 MOD_RES 49 49 Phosphoserine; by HRI (By similarity).
 MOD_RES 52 52 Phosphoserine; by EIF2AK3, GCN2, HRI and
 MOD_RES 141 141 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 315 AA 

Protein Sequence

Gene Ontology

 GO:0010494; C:cytoplasmic stress granule; IDA:UniProtKB.
 GO:0005850; C:eukaryotic translation initiation factor 2 complex; IEA:InterPro.
 GO:0005851; C:eukaryotic translation initiation factor 2B complex; IEA:Compara.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0043022; F:ribosome binding; ISS:UniProtKB.
 GO:0003743; F:translation initiation factor activity; ISS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:MGI.
 GO:0043558; P:regulation of translational initiation in response to stress; IDA:MGI. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR003029; Rbsml_prot_S1_RNA-bd_dom.
 IPR022967; RNA-binding_domain_S1.
 IPR024055; TIF2_asu_C.
 IPR024054; TIF2_asu_middle.
 IPR011488; TIF_2_asu. 

Pfam

 PF07541; EIF_2_alpha
 PF00575; S1 

SMART

 SM00316; S1 

PROSITE

 PS50126; S1 

PRINTS