CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020315
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bardet-Biedl syndrome 2 protein 
Protein Synonyms/Alias
  
Gene Name
 BBS2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9LLPVFTLKLRHKISPubiquitination[1]
39AAATQTGKVFIHNPHubiquitination[1]
231GTVGVYDKTSRYWRIubiquitination[1, 2, 3]
268ITGWSNGKVDARSDRubiquitination[1]
345IRELSQKKQNLLLELubiquitination[1, 4]
360RNYEENAKAELASPLubiquitination[1, 2, 3]
599ELRKVLVKVDEYHSVubiquitination[1]
609EYHSVHQKLSADMADubiquitination[1, 2, 3, 5, 6]
659RDLLNGYKIRCNNHTubiquitination[1, 2, 5, 6]
673TELLGNLKAVNQAIQubiquitination[1]
689AGRLRVGKPKNQVITubiquitination[1]
691RLRVGKPKNQVITACubiquitination[1]
712NNINTLFKIMRVGTAubiquitination[5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. 
Sequence Annotation
  
Keyword
 Bardet-Biedl syndrome; Cell membrane; Cell projection; Ciliopathy; Cilium; Coiled coil; Complete proteome; Cytoplasm; Disease mutation; Membrane; Mental retardation; Obesity; Polymorphism; Reference proteome; Sensory transduction; Vision. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 721 AA 
Protein Sequence
MLLPVFTLKL RHKISPRMVA IGRYDGTHPC LAAATQTGKV FIHNPHTRNQ HVSASRVFQS 60
PLESDVSLLN INQAVSCLTA GVLNPELGYD ALLVGTQTNL LAYDVYNNSD LFYREVADGA 120
NAIVLGTLGD ISSPLAIIGG NCALQGFNHE GSDLFWTVTG DNVNSLALCD FDGDGKKELL 180
VGSEDFDIRV FKEDEIVAEM TETEIVTSLC PMYGSRFGYA LSNGTVGVYD KTSRYWRIKS 240
KNHAMSIHAF DLNSDGVNEL ITGWSNGKVD ARSDRTGEVI FKDNFSSAIA GVVEGDYRMD 300
GHIQLICCSV DGEIRGYLPG TAEMRGNLMD TSAEQDLIRE LSQKKQNLLL ELRNYEENAK 360
AELASPLNEA DGHRGIIPAN TRLHTTLSVS LGNETQTAHT ELRISTSNDT IIRAVLIFAE 420
GIFTGESHVV HPSIHNLSSS ICIPIVPPKD VPVDLHLKAF VGYRSSTQFH VFESTRQLPR 480
FSMYALTSLD PASEPISYVN FTIAERAQRV VVWLGQNFLL PEDTHIQNAP FQVCFTSLRN 540
GGHLHIKIKL SGEITINTDD IDLAGDIIQS MASFFAIEDL QVEADFPVYF EELRKVLVKV 600
DEYHSVHQKL SADMADHSNL IRSLLVGAED ARLMRDMKTM KSRYMELYDL NRDLLNGYKI 660
RCNNHTELLG NLKAVNQAIQ RAGRLRVGKP KNQVITACRD AIRSNNINTL FKIMRVGTAS 720
S 721 
Gene Ontology
 GO:0034464; C:BBSome; IDA:BHF-UCL.
 GO:0060170; C:cilium membrane; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005932; C:microtubule basal body; IDA:BHF-UCL.
 GO:0031514; C:motile cilium; IDA:BHF-UCL.
 GO:0030534; P:adult behavior; ISS:BHF-UCL.
 GO:0048854; P:brain morphogenesis; ISS:BHF-UCL.
 GO:0021987; P:cerebral cortex development; ISS:BHF-UCL.
 GO:0045444; P:fat cell differentiation; ISS:BHF-UCL.
 GO:0043001; P:Golgi to plasma membrane protein transport; IMP:MGI.
 GO:0021766; P:hippocampus development; ISS:BHF-UCL.
 GO:0032402; P:melanosome transport; ISS:BHF-UCL.
 GO:0038108; P:negative regulation of appetite by leptin-mediated signaling pathway; ISS:BHF-UCL.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0040015; P:negative regulation of multicellular organism growth; ISS:BHF-UCL.
 GO:0045494; P:photoreceptor cell maintenance; ISS:BHF-UCL.
 GO:0040018; P:positive regulation of multicellular organism growth; IEA:Compara.
 GO:0033365; P:protein localization to organelle; ISS:BHF-UCL.
 GO:0060296; P:regulation of cilium beat frequency involved in ciliary motility; ISS:BHF-UCL.
 GO:0007288; P:sperm axoneme assembly; ISS:BHF-UCL.
 GO:0021756; P:striatum development; ISS:BHF-UCL. 
Interpro
 IPR016616; Bardet-Biedl_syndrome_2_prot.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR017986; WD40_repeat_dom. 
Pfam
  
SMART
  
PROSITE
  
PRINTS