UniProt Accession

 PFLB_ECOLI; P09373; P76826; Q47478 

Genbank Protein ID

 X08035; U00096; AP009048; M26413 

Genbank Nucleotide ID

 CAA30828.1; AAC73989.1; BAA35638.1; AAA20391.1 

Protein Name

 Formate acetyltransferase 1 

Protein Synonyms/Alias

 Pyruvate formate-lyase 1 

Gene Name

 pflB 

Gene Synonyms/Alias

 pfl; b0903; JW0886 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
7	*MSELNEKLATAWEG	acetylation	[1]
17	TAWEGFTKGDWQNEV	acetylation	[2]
32	NVRDFIQKNYTPYEG	acetylation	[2]
63	DKVMEGVKLENRTHA	acetylation	[1, 2, 3]
92	HDAGYINKQLEKIVG	acetylation	[2]
96	YINKQLEKIVGLQTE	acetylation	[2]
107	LQTEAPLKRALIPFG	acetylation	[2]
117	LIPFGGIKMIEGSCK	acetylation	[1, 2, 3]
124	KMIEGSCKAYNRELD	acetylation	[1, 2]
135	RELDPMIKKIFTEYR	acetylation	[2]
136	ELDPMIKKIFTEYRK	acetylation	[1, 2]
162	PDILRCRKSGVLTGL	acetylation	[1, 2]
195	YGIDYLMKDKLAQFT	acetylation	[1, 2, 3]
197	IDYLMKDKLAQFTSL	acetylation	[1, 2]
235	HRALGQMKEMAAKYG	acetylation	[1, 2]
293	VYIERDLKAGKITEQ	acetylation	[2]
312	MVDHLVMKLRMVRFL	acetylation	[1, 2]
352	DGRTLVTKNSFRFLN	acetylation	[2]
379	MTILWSEKLPLNFKK	acetylation	[2]
385	EKLPLNFKKFAAKVS	acetylation	[2]
441	GARANLAKTMLYAIN	acetylation	[2]
454	INGGVDEKLKMQVGP	acetylation	[1, 2, 3]
456	GGVDEKLKMQVGPKS	acetylation	[1, 2]
462	LKMQVGPKSEPIKGD	acetylation	[1, 2]
467	GPKSEPIKGDVLNYD	acetylation	[1, 2]
541	ADSLSAIKYAKVKPI	acetylation	[2, 3]
544	LSAIKYAKVKPIRDE	acetylation	[1]
588	LVERFMKKIQKLHTY	acetylation	[2]
591	RFMKKIQKLHTYRDA	acetylation	[1, 2, 3]
615	TSNVVYGKKTGNTPD	acetylation	[2]
643	PMHGRDQKGAVASLT	acetylation	[2]
654	ASLTSVAKLPFAYAK	acetylation	[1, 2]
661	KLPFAYAKDGISYTF	acetylation	[2]
677	IVPNALGKDDEVRKT	acetylation	[2]
725	DAMENPEKYPQLTIR	acetylation	[2]
745	VRFNSLTKEQQQDVI	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

  

Sequence Annotation

 DOMAIN 3 625 PFL.
 DOMAIN 632 760 Glycine radical.
 ACT_SITE 419 419 S-acetylcysteine intermediate.
 ACT_SITE 420 420 Cysteine radical intermediate.
 MOD_RES 63 63 N6-acetyllysine; alternate.
 MOD_RES 63 63 N6-succinyllysine; alternate.
 MOD_RES 107 107 N6-succinyllysine.
 MOD_RES 117 117 N6-acetyllysine; alternate.
 MOD_RES 117 117 N6-succinyllysine; alternate.
 MOD_RES 124 124 N6-acetyllysine; alternate.
 MOD_RES 124 124 N6-succinyllysine; alternate.
 MOD_RES 195 195 N6-acetyllysine; alternate.
 MOD_RES 195 195 N6-succinyllysine; alternate.
 MOD_RES 454 454 N6-acetyllysine; alternate.
 MOD_RES 454 454 N6-succinyllysine; alternate.
 MOD_RES 467 467 N6-succinyllysine.
 MOD_RES 541 541 N6-acetyllysine.
 MOD_RES 591 591 N6-acetyllysine.
 MOD_RES 654 654 N6-succinyllysine.
 MOD_RES 735 735 Glycine radical.  

Keyword

 3D-structure; Acetylation; Acyltransferase; Carbohydrate metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Glucose metabolism; Organic radical; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 760 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0008861; F:formate C-acetyltransferase activity; IDA:EcoCyc.
 GO:0009061; P:anaerobic respiration; IEP:EcoCyc.
 GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW. 

Interpro

 IPR005949; Form_AcTrfase.
 IPR019777; Form_AcTrfase_GR_CS.
 IPR001150; Gly_radical.
 IPR004184; PFL_dom. 

Pfam

 PF01228; Gly_radical
 PF02901; PFL 

SMART

  

PROSITE

 PS00850; GLY_RADICAL_1
 PS51149; GLY_RADICAL_2
 PS51554; PFL 

PRINTS