UniProt Accession

 OTUB2_HUMAN; Q96DC9; Q6IA10; Q9H6T1 

Genbank Protein ID

 AY177201; AK025569; CR457345; AL079302; BC009615; BC068058 

Genbank Nucleotide ID

 AAO27703.1; BAB15172.1; CAG33626.1; AAH09615.1; AAH68058.1 

Protein Name

 Ubiquitin thioesterase OTUB2 

Protein Synonyms/Alias

 Deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin-specific-processing protease OTUB2 

Gene Name

 OTUB2 

Gene Synonyms/Alias

 C14orf137; OTB2; OTU2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
94	AAGFEEHKFRNFFNA	ubiquitination	[1, 2, 3, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. 

Sequence Annotation

 DOMAIN 40 231 OTU.
 ACT_SITE 48 48 By similarity.
 ACT_SITE 51 51 Nucleophile (Probable).
 ACT_SITE 224 224  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 234 AA 

Protein Sequence

Gene Ontology

 GO:0008242; F:omega peptidase activity; IEA:InterPro.
 GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB.
 GO:0006520; P:cellular amino acid metabolic process; IDA:UniProtKB.
 GO:0071108; P:protein K48-linked deubiquitination; IDA:UniProtKB.
 GO:0070536; P:protein K63-linked deubiquitination; IDA:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR003323; OTU.
 IPR019400; Peptidase_C65_otubain.
 IPR016615; Ubiquitin_thioesterase_Otubain. 

Pfam

 PF10275; Peptidase_C65 

SMART

  

PROSITE

 PS50802; OTU 

PRINTS