CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017047
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-conjugating enzyme E2 variant 3 
Protein Synonyms/Alias
 UEV-3; EV and lactate/malate dehydrogenase domain-containing protein 
Gene Name
 UEVLD 
Gene Synonyms/Alias
 UEV3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45SMDTYVFKDSSQKDLubiquitination[1, 2, 3]
172GVSDTNSKSWANHENubiquitination[4]
341LKAQTSGKEVWVIGEubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Possible negative regulator of polyubiquitination. 
Sequence Annotation
 DOMAIN 2 145 UEV.
 NP_BIND 191 219 NAD (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; NAD; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 471 AA 
Protein Sequence
MEFDCEGLRR LLGKYKFRDL TVEELRNVNV FFPHFKYSMD TYVFKDSSQK DLLNFTGTIP 60
VMYQGNTYNI PIRFWILDSH PFAPPICFLK PTANMGILVG KHVDAQGRIY LPYLQNWSHP 120
KSVIVGLIKE MIAKFQEELP MYSLSSSDEA RQVDLLAYIA KITEGVSDTN SKSWANHENK 180
TVNKITVVGG GELGIACTLA ISAKGIADRL VLLDLSEGTK GATMDLEIFN LPNVEISKDL 240
SASAHSKVVI FTVNSLGSSQ SYLDVVQSNV DMFRALVPAL GHYSQHSVLL VASQPVEIMT 300
YVTWKLSTFP ANRVIGIGCN LDSQRLQYII TNVLKAQTSG KEVWVIGEQG EDKVLTWSGQ 360
EEVVSHTSQV QLSNRAMELL RVKGQRSWSV GLSVADMVDS IVNNKKKVHS VSALAKGYYD 420
INSEVFLSLP CILGTNGVSE VIKTTLKEDT VTEKLQSSAS SIHSLQQQLK L 471 
Gene Ontology
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006464; P:cellular protein modification process; IEA:InterPro.
 GO:0015031; P:protein transport; IEA:InterPro. 
Interpro
 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR016040; NAD(P)-bd_dom.
 IPR016135; UBQ-conjugating_enzyme/RWD.
 IPR008883; UEV_N. 
Pfam
 PF02866; Ldh_1_C
 PF00056; Ldh_1_N
 PF05743; UEV 
SMART
  
PROSITE
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2
 PS51322; UEV 
PRINTS
 PR00086; LLDHDRGNASE.