UniProt Accession

 DNJA1_MOUSE; P63037; P54102 

Genbank Protein ID

 AF055664; AK083046; BC057876 

Genbank Nucleotide ID

 AAC78597.1; BAC38744.1; AAH57876.1 

Protein Name

 DnaJ homolog subfamily A member 1 

Protein Synonyms/Alias

 DnaJ protein homolog 2; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2 

Gene Name

 Dnaja1 

Gene Synonyms/Alias

 Dnaj2; Hsj2; Hspf4 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
3	*****MVKETTYYDV	ubiquitination	[1]
14	YYDVLGVKPNATQEE	ubiquitination	[1]
23	NATQEELKKAYRKLA	ubiquitination	[1]
32	AYRKLALKYHPDKNP	ubiquitination	[1]
37	ALKYHPDKNPNEGEK	ubiquitination	[1]
44	KNPNEGEKFKQISQA	ubiquitination	[1]
46	PNEGEKFKQISQAYE	ubiquitination	[1]
59	YEVLADSKKRELYDK	ubiquitination	[1]
66	KKRELYDKGGEQAIK	ubiquitination	[1]
136	QKNVICDKCEGRGGK	acetylation	[2]
296	SHPGQIVKHGDIKCV	ubiquitination	[1]
338	NGFLSPDKLSLLEKL	ubiquitination	[1]
344	DKLSLLEKLLPERKE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria (By similarity). 

Sequence Annotation

 DOMAIN 6 68 J.
 REPEAT 134 141 CXXCXGXG motif.
 REPEAT 150 157 CXXCXGXG motif.
 REPEAT 177 184 CXXCXGXG motif.
 REPEAT 193 200 CXXCXGXG motif.
 ZN_FING 121 205 CR-type.
 METAL 134 134 Zinc 1 (By similarity).
 METAL 137 137 Zinc 1 (By similarity).
 METAL 150 150 Zinc 2 (By similarity).
 METAL 153 153 Zinc 2 (By similarity).
 METAL 177 177 Zinc 2 (By similarity).
 METAL 180 180 Zinc 2 (By similarity).
 METAL 193 193 Zinc 1 (By similarity).
 METAL 196 196 Zinc 1 (By similarity).
 MOD_RES 66 66 N6-acetyllysine (By similarity).
 MOD_RES 83 83 Phosphoserine (By similarity).
 MOD_RES 335 335 Phosphoserine (By similarity).
 MOD_RES 381 381 Phosphotyrosine (By similarity).
 MOD_RES 394 394 Cysteine methyl ester (By similarity).
 LIPID 394 394 S-farnesyl cysteine (By similarity).  

Keyword

 Acetylation; Chaperone; Complete proteome; Direct protein sequencing; Lipoprotein; Membrane; Metal-binding; Methylation; Phosphoprotein; Prenylation; Reference proteome; Repeat; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 397 AA 

Protein Sequence

Gene Ontology

 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0050750; F:low-density lipoprotein particle receptor binding; ISO:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0030521; P:androgen receptor signaling pathway; IMP:MGI.
 GO:0042769; P:DNA damage response, detection of DNA damage; IEA:Compara.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0009408; P:response to heat; IEA:InterPro.
 GO:0030317; P:sperm motility; IMP:MGI.
 GO:0007283; P:spermatogenesis; IMP:MGI. 

Interpro

 IPR012724; DnaJ.
 IPR002939; DnaJ_C.
 IPR001623; DnaJ_domain.
 IPR018253; DnaJ_domain_CS.
 IPR008971; HSP40/DnaJ_pept-bd.
 IPR001305; HSP_DnaJ_Cys-rich_dom. 

Pfam

 PF00226; DnaJ
 PF01556; DnaJ_C
 PF00684; DnaJ_CXXCXGXG 

SMART

 SM00271; DnaJ 

PROSITE

 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS51188; ZF_CR 

PRINTS

 PR00625; JDOMAIN.