CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014810
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell division cycle 5-like protein 
Protein Synonyms/Alias
 Cdc5-like protein 
Gene Name
 Cdc5l 
Gene Synonyms/Alias
 Kiaa0432 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
294EFTKKRSKLVLPAPQubiquitination[1]
466YSDPSYVKQMERESRubiquitination[1]
771PRRLECLKEDVQRQQacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing (By similarity). 
Sequence Annotation
 DOMAIN 1 56 HTH myb-type 1.
 DOMAIN 57 108 HTH myb-type 2.
 DNA_BIND 31 54 H-T-H motif (By similarity).
 DNA_BIND 82 104 H-T-H motif (By similarity).
 REGION 165 271 Nuclear localization signal (Potential).
 REGION 200 206 Required for interaction with CTNNBL1 (By
 REGION 260 606 Interaction with PPP1R8 (By similarity).
 REGION 501 659 Interaction with DAPK3 (By similarity).
 REGION 706 800 Interaction with PLRG1 (By similarity).
 MOD_RES 227 227 Phosphothreonine (By similarity).
 MOD_RES 303 303 Phosphoserine (By similarity).
 MOD_RES 358 358 Phosphoserine (By similarity).
 MOD_RES 377 377 Phosphothreonine (By similarity).
 MOD_RES 385 385 Phosphothreonine.
 MOD_RES 396 396 Phosphothreonine.
 MOD_RES 404 404 Phosphothreonine (By similarity).
 MOD_RES 411 411 Phosphothreonine (By similarity).
 MOD_RES 415 415 Phosphothreonine (By similarity).
 MOD_RES 417 417 Phosphoserine (By similarity).
 MOD_RES 424 424 Phosphothreonine (By similarity).
 MOD_RES 430 430 Phosphothreonine (By similarity).
 MOD_RES 438 438 Phosphothreonine (By similarity).
 MOD_RES 442 442 Phosphothreonine (By similarity).  
Keyword
 Activator; Cell cycle; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Spliceosome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 802 AA 
Protein Sequence
MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK 60
TEWSREEEEK LLHLAKLMPT QWRTIAPIIG RTAAQCLEHY EFLLDKTAQR DNEEETTDDP 120
RKLKPGEIDP NPETKPARPD PIDMDEDELE MLSEARARLA NTQGKKAKRK AREKQLEEAR 180
RLAALQKRRE LRAAGIEIQK KRKKKRGVDY NAEIPFEKKP ALGFYDTSEE NYQALDADFR 240
KLRQQDLDGE LRSEKEGRDR KKDKQHLKRK KESDLPSAIL QTSGVSEFTK KRSKLVLPAP 300
QISDAELQEV VKVGQASEVA RQTAEESGIT NSASSTLLSE YNVTNNSIAL RTPRTPASQD 360
RILQEAQNLM ALTNVDTPLK GGLNTPLHES DFSGVTPQRQ VVQTPNTVLS TPFRTPSNGA 420
EGLTPRSGTT PKPVTNATPG RTPLRDKLNI NPEDGMADYS DPSYVKQMER ESREHLRLGL 480
LGLPAPKNDF EIVLPENAEK ELEEREIDDT YIEDAADVDA RKQAIRDAER VKEMKRMHKA 540
VQKDLPRPSE VNETILRPLN VEPPLTDLQK SEELIKKEMI TMLHYDLLHH PYEPSGNKKG 600
KNVGFATNNS EHITYLEHSP YEKFSKEDLK KAQDALVQEM EVVKQGMSHG ELSSEAYNQV 660
WEECYSQVLY LPAQSRYTRA NLASKKDRIE SLEKRLEINR GHMTTEAKRA AKMEKKMKIL 720
LGGYQSRAMG LMKQLNDLWD QIEQAHLELR TFEELKKHED SAIPRRLECL KEDVQRQQER 780
EKELQQRYAD LLMEKETLQA KF 802 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0000974; C:Prp19 complex; ISS:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0071987; F:WD40-repeat domain binding; ISS:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR021786; DUF3351.
 IPR009057; Homeodomain-like.
 IPR017930; Myb_dom.
 IPR001005; SANT/Myb. 
Pfam
 PF11831; Myb_Cef 
SMART
 SM00717; SANT 
PROSITE
 PS51294; HTH_MYB 
PRINTS