CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024352
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase UBR4 
Protein Synonyms/Alias
 N-recognin-4; Zinc finger UBR1-type protein 1; p600 
Gene Name
 Ubr4 
Gene Synonyms/Alias
 Kiaa0462; Zubr1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
187VSPELRQKEVQMNFLubiquitination[1]
634NKQASGEKGNILASRubiquitination[1]
1149AAETDPHKSSEITKNubiquitination[1]
1573CKKYLSQKNVVEKLNubiquitination[1]
4047MCLRILQKLIKPPAPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. 
Sequence Annotation
 ZN_FING 1655 1728 UBR-type.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 1083 1083 N6-acetyllysine (By similarity).
 MOD_RES 1401 1401 Phosphoserine (By similarity).
 MOD_RES 2712 2712 Phosphothreonine.
 MOD_RES 2716 2716 Phosphoserine.
 MOD_RES 2719 2719 Phosphoserine.
 MOD_RES 2721 2721 Phosphothreonine.  
Keyword
 Acetylation; Alternative splicing; Calmodulin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Ligase; Membrane; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 5180 AA 
Protein Sequence
MATSGGEEAA AAAPAPGAPA TGQDTTPGWE VAVRPLLSAS YSAFEMKELP QLVASVIESE 60
SEILHHEKQY EPFYSSFVAL STHYITTVCS LIPRNQLQSV AAACKVLIEF SLLRLENPDE 120
ACAVSQKHLI LLIKGLCTGC SRLDRTEIIT FTAMMKSAKL PQTVKTLSDV EDQKELASPV 180
SPELRQKEVQ MNFLNQLTSV FNPRTVPSPP ISPQALVEGE NDEQSSPDQV SAAKTKSVFI 240
AQNVASLQEL GGSEKLLRVC LNLPYFLRYI NRFQDAVVAN SFFIMPATVA DATAVRNGFH 300
SLVIDVTMAL DTLSLPVLEP LNPSRLQDVT VLSLSCLYAG VSVATCMAIL HVGSAQQVRT 360
GSTSSKEDDY ESDAATIVQK CLEIYDMIGQ AISSSRRAGG EHFQNFQLLG AWCLLNSLFL 420
ILNLSPTALA DKGKEKDPLA ALRVRDILSR TKEGVGSPKL GPGKGHQGFG VLSVILANHA 480
IKLLASLFQD LQVEALHKGW ETDGPPAVLS IMAQSTSTQR IQRLIDSVPL TNLLLTLLST 540
SYRKACVLQR QRKGSMSSDA SASTDSNTYY EDDFSSTEED SSQDDDSEPI LGQWFEETIS 600
PSKEKAAPPP PPPPPPLESS PRVKSPNKQA SGEKGNILAS RKDPELFSGL ASNILNFITT 660
SMLNSRNSFI RSYLSASLSE HHMATLASII KEVDKDGLKG SSDEDFAAAL YHFNHSLVTS 720
DLQSPNLQNT LLQQLGVAPF SEGPWPLYIH PQGLSVLSRL LLIWQHKAGA QGDPDVPECL 780
KVWDRFLTTM KQNALQGVVP SETEDLNVEH LQLLLLIFHS FSEKGRRAIL TMLVQSIQEL 840
SVNMEVQMRT APLILARLLL IFDYLLHQYS KAPVYLFEQV QHNLLSPPFG WASGSQDSSS 900
RRANTPLYHG FKEVEENWSK HFSSDAAPQP RFYCVLSTEA SEEDLNRLDS EACEVLFSKP 960
VKYDELYSSL TTLLAAGSQL DTTRRKEKKN VTALEACALQ YYFLILWRIL GILPPSKTYM 1020
NQLAMNSPEM SECDILHTLR WSSRLRISSY VSWIKDHLIK QGMKPEHAGS LIELAASKCS 1080
SVKYDVEIVE EYFARQISSF CSIDCTAVLQ LHEIPSLQSI YTLDAAVSKV QVSLDEHFSK 1140
MAAETDPHKS SEITKNLLPA TLQLIDTYAS FTRAYLLQNL NEEGSTEKPS QEKLHGFAAV 1200
LAIGSSRCKA NTLGPTLVQN LPSSVQSVCE SWNNINTNEF PNIGSWRNAF ANDTIPSESY 1260
ISAVQAAHLG TLCGQSLPLA ASLKHTLLSL VRLTGDLIVW SDEMNPAQVI RTLLPLLLES 1320
STESAAEISS NSLERILGPA ESDEFLARVY EKLITGCYNI LANHADPNSG LDESILEECL 1380
QYLEKQLESS QARKAMEEFF SDGGELVQIM MATANEDLSA KFCNRVLKFF TKLFQLTEKS 1440
PNPSLLHLCG SLAQLACVEP VRLQAWLTRM TTSPPKDSDQ LEVIQENRQL LQLLTTYIVR 1500
ENSQVGEGVC AVLLGTLTPM ATDMLANGDG TGFPELMVVM ATLASAGQGA GHLQLHNAAV 1560
DWLGRCKKYL SQKNVVEKLN ANVMHGKHVM VLECTCHIMS YLADVTNALS QSNGQGPSHL 1620
SVDGEERAIE VDSDWVEELA VEEEDSQAED SDEDSLCNKL CTFTITQKEF MNQHWYHCHT 1680
CKMVDGVGVC TVCAKVCHKD HEISYAKYGS FFCDCGAKED GSCLALVKRT PSSGMSSTMK 1740
ESAFQSEPRV SESLVRHAST SPADKAKVTI SDGKVTDEEK PKKSSLCRTV EGCREELQNQ 1800
ANFSFAPLVL DMLSFLMDAI QTNFQQASAV GSSSRAQQAL SELHTVDKGV EMTDQLMVPT 1860
LGSQEGAFEN VRMNYSGDQG QTIRQLISAH VLRRVAMCVL SSPHGRRQHL AVSHEKGKIT 1920
VLQLSALLKQ ADSSKRKLTL TRLASAPVPF TVLSLTGNPC KEDYLAVCGL KDCHVLTFSS 1980
SGSVSDHLVL HPQLATGNFI IKAVWLPGSQ TELAIVTADF VKIYDLSIDA LSPTFYFLLP 2040
SSKIRDVTFL FNEEGKNIIV IMSSAGYMYT QLMEEASSAQ QGPFYVTNVL EINHEDLKDS 2100
NSQVAGGGVS VYYSHVLQML FFSYSQGRSF AATVSRSTLE VLQLFPINIK SSNGGSKTSP 2160
ALCQWSEVMN HPGLVCCVQQ TTGVPLVVMV KPGTFLIQEI KTLPAKAKIQ DMVAIRHTAC 2220
NEQQRTTMIL LCEDGSLRIY MANVENTSYW LQPSLQPSSV ISIMKPVRKR KTATITARTS 2280
SQVTFPIDFF EHNQQLTDVE FGGNDLLQVY NAQQIKHRLN STGMYVANTK PGGFTIEISN 2340
NSSTMVMTGM RIQIGTQAIE RAPSYIEIFG RTMQLNLSRS RWFDFPFTRE EALQADRKLS 2400
LFIGASVDPA GVTMIDAVKI YGKTKEQFGW PDEPPEDFPS ASVSNICPPN LNQSNGTGES 2460
DSAAPATTSG TVLERLVVSS LEALESCFAV GPIIEKERNK HAAQELATLL LSLPAPASVQ 2520
QQSKSLLASL HSSRSAYHSH KDQALLSKAV QCLNTSSKEG KDLDPEVFQR LVITARSIAV 2580
TRPNNLVHFT ESKLPQMETD CFSPRCACWN LGIVGILIGA PLETPSAEGA DEGKEPQKQE 2640
GDGCSFITQL VNHFWKLHAS KPKNAFLAPA CLPGLTHIEA TVNALVDIIH GYCTCELDCI 2700
NTASKIYMQM LLCPDPAVSF SCKQALIRVL RPRNKRRHVT LPSSPRSNTP MGDKDDDDDD 2760
DADEKMQSSG IPDGGHIRQE SQEQSEVDHG DFEMVSESMV LETAENVNNG NPSPLEALLA 2820
GAEGFPPMLD IPPDADDETM VELAIALSLQ QDQQGSSSSA LGLQSLGLSG QAPSSSSLDA 2880
GTLSDTTASA PASDDEGSTA ATDGSTLRTS PADHGGSVGS ESGGSAVDSV AGEHSVSGRS 2940
SAYGDATAEG HPAGPGSVSS STGAISTATG HQEGDGSEGE GEGEAEGDVH TSNRLHMVRL 3000
MLLERLLQTL PQLRNVGGVR AIPYMQVILM LTTDLDGEDE KDKGALDNLL AQLIAELGMD 3060
KKDVSKKNER SALNEVHLVV MRLLSVFMSR TKSGSKSSIC ESSSLISSAT AAALLSSGAV 3120
DYCLHVLKSL LEYWKSQQSD EEPVAASQLL KPHTTSSPPD MSPFFLRQYV KGHAADVFEA 3180
YTQLLTEMVL RLPYQIKKIA DTSSRIPPPV FDHSWFYFLS EYLMIQQTPF VRRQVRKLLL 3240
FICGSKEKYR QLRDLHTLDS HVRGIKKLLE EQGIFLRASV VTASSGSALQ YDTLISLMEH 3300
LKACAEIAAQ RTINWQKFCI KDDSVLYFLL QVSFLVDEGV SPVLLQLLSC ALCGSKVLAA 3360
LAASTGSSSV ASSSAPPAAS SGQATTQSKS STKKSKKEEK EKEKEGESSG SQEDQLCTAL 3420
VNQLNRFADK ETLIQFLRCF LLESNSSSVR WQAHCLTLHI YRNSNKAQQE LLLDLMWSIW 3480
PELPAYGRKA AQFVDLLGYF SLKTAQTEKK LKEYSQKAVE ILRTQNHILT NHPNSNIYNT 3540
LSGLVEFDGY YLESDPCLVC NNPEVPFCYI KLSSIKVDTR YTTTQQVVKL IGSHTISKVT 3600
VKIGDLKRTK MVRTINLYYN NRTVQAIVEL KNKPARWHKA KKVQLTPGQT EVKIDLPLPI 3660
VASNLMIEFA DFYENYQAST ETLQCPRCSA SVPANPGVCG NCGENVYQCH KCRSINYDEK 3720
DPFLCNACGF CKYARFDFML YAKPCCAVDP IENEEDRKKA VSNINTLLDK ADRVYHQLMG 3780
HRPQLENLLC KVNEAAPEKP QEDSGTAGGI SSTSASVNRY ILQLAQEYCG DCKNSFDELS 3840
KIIQKVFASR KELLEYDLQQ REAATKSSRT SVQPTFTASQ YRALSVLGCG HTSSTKCYGC 3900
ASAVTEHCIT LLRALATNPA LRHILVSQGL IRELFDYNLR RGAAAIREEV RQLMCLLTRD 3960
NPEATQQMND LIIGKVSTAL KGHWANPDLA SSLQYEMLLL TDSISKEDSC WELRLRCALS 4020
LFLMAVNIKT PVVVENITLM CLRILQKLIK PPAPTSKKNK DVPVEALTTV KPYCNEIHAQ 4080
AQLWLKRDPK ASYEAWKKCL PIRGVDGNGK SPSKSELHRL YLTEKYVWRW KQFLSRRGKR 4140
TTPLDLKLGH NNWLRQVLFT PATQAARQAA CTIVEALATV PSRKQQVLDL LTSYLDELSV 4200
AGECAAEYLA LYQKLIASCH WKVYLAARGV LPYVGNLITK EIARLLALEE ATLSTDLQQG 4260
YALKSLTGLL SSFVEVESIK RHFKSRLVGT VLNGYLCLRK LVLQRTKLID ETQDMLLEML 4320
EDMTTGTESE TKAFMAVCIE TAKRYNLDDY RTPVFIFERL CSIIYPEENE VTEFFVTLEK 4380
DPQQEDFLQG RMPGNPYSSN EPGIGPLMRD IKNKICQDCD LVALLEDDSG MELLVNNKII 4440
SLDLPVAEVY KKVWCATNEG EPMRIVYRMR GLLGDATEEF IESLDSTTDE EEDEEEVYRM 4500
AGVMAQCGGL QCMLNRLAGV KDFKQGRHLL TVLLKLFSYC VKVKVNRQQL VKLETNTLNV 4560
MLGTLNLALV AEQESKDSGG AAVAEQVLSI MEIILDESNA EPLSEDKGNL LLTGDKDQLV 4620
MLLDQINSTF VRSNPSVLQG LLRIIPYLSF GEVEKMQILV ERFKPYCSFE KYDEDHSGDD 4680
KVFLDCFCKI AAGIKNNSNG HQLKDLILQK GITQNALDYM KKHIPSAKNL DADIWKKFLS 4740
RPALPFILRL LRGLAMQHPA TQVLIGTDSI TSLHKLEQVS SDEGIGTLAE NLLEALREHP 4800
DVNKKIDAAR RETRAEKKRM AMAMRQKALG TLGMTTNEKG QVVTKTALLK QMEELIEEPG 4860
LTCCICREGY KFQPTKVLGI YTFTKRVALE EMENKPRKQQ GYSTVSHFNI VHYDCHLAAV 4920
RLARGREEWE SAALQNANTK CNGLLPVWGP HVPESAFATC LARHNTYLQE CTGQREPTYQ 4980
LNIHDIKLLF LRFAMEQSFS ADTGGGGRES NIHLIPYIIH TVLYVLNTTR ATSREEKNLQ 5040
GFLEQPKEKW TESAFDVDGP HYFTILALHV LPPEQWKAIR VEILRRLLVA SHARAVAPGG 5100
ATRLTDKAVK DYSAYRSSLL FWALVDLIYN MFKKVPTSNT EGGWSCSLAE YIRHNDMPIY 5160
EAADKALKTF QEEFMPVETF SEFLDAAGLL SEITDPESFL KDLLNSVP 5208 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR025704; E3_Ub_ligase_UBR4.
 IPR003126; Znf_N-recognin. 
Pfam
 PF13764; E3_UbLigase_R4
 PF02207; zf-UBR 
SMART
  
PROSITE
 PS51157; ZF_UBR 
PRINTS