CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016386
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Enolase-phosphatase E1 
Protein Synonyms/Alias
 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MASA homolog 
Gene Name
 Enoph1 
Gene Synonyms/Alias
 Masa 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
36DVLFPYIKENVKEYLacetylation[1]
36DVLFPYIKENVKEYLubiquitination[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) (By similarity). 
Sequence Annotation
 REGION 150 151 Substrate binding (By similarity).
 METAL 16 16 Magnesium (By similarity).
 METAL 18 18 Magnesium; via carbonyl oxygen (By
 METAL 209 209 Magnesium (By similarity).
 BINDING 184 184 Substrate (By similarity).  
Keyword
 Alternative splicing; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Hydrolase; Magnesium; Metal-binding; Methionine biosynthesis; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 257 AA 
Protein Sequence
MVVVSVPAEV TVILLDIEGT TTPIAFVKDV LFPYIKENVK EYLQTHWEEE ECQQDVSLLR 60
KQAEEDAHLD GAVPIPVASG SDLQQMIQAV VDNVYWQMSH DRKTTALKQL QGHMWKAAFT 120
AGRMKAEFFA DVVPAVRRWR EAGMKVYIYS SGSVEAQKLL FGHSTEGDIL ELIDGHFDTK 180
IGHKVDSESY RKIADSIGCS TNNILFLTDV TVEASAAEEA DVHVAVVVRP GNAGLTDDEK 240
TYYNLITSFS ELYLPST 257 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0043874; F:acireductone synthase activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0008967; F:phosphoglycolate phosphatase activity; IEA:InterPro.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; ISS:UniProtKB. 
Interpro
 IPR023943; Enolase-ppase_E1.
 IPR027511; ENOPH1_eukaryotes.
 IPR023214; HAD-like_dom.
 IPR006439; HAD-SF_hydro_IA_v1. 
Pfam
 PF13419; HAD_2 
SMART
  
PROSITE
  
PRINTS