| Tag | Content |
|---|
| CPLM ID | CPLM-003506 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | 5,10-methylenetetrahydrofolate reductase |
Protein Synonyms/Alias | |
Gene Name | metF |
Gene Synonyms/Alias | b3941; JW3913 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 52 | IDRLSSLKPKFVSVT | acetylation | [1] | | 54 | RLSSLKPKFVSVTYG | acetylation | [1] | | 75 | DRTHSIIKGIKDRTG | acetylation | [1] | | 127 | DLPPGSGKPEMYASD | acetylation | [1] | | 160 | PEVHPEAKSAQADLL | acetylation | [1] | | 170 | QADLLNLKRKVDAGA | acetylation | [1] | | 221 | VSNFKQAKKFADMTN | acetylation | [1] | | 222 | SNFKQAKKFADMTNV | acetylation | [1] | | 262 | NIAMDMVKILSREGV | acetylation | [1] | | 270 | ILSREGVKDFHFYTL | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Methylenetetrahydrofolate reductase required to generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine. |
Sequence Annotation | NP_BIND 59 62 FAD.
NP_BIND 118 120 FAD.
NP_BIND 131 133 FAD.
NP_BIND 156 159 FAD.
NP_BIND 165 172 FAD.
BINDING 88 88 FAD.
BINDING 152 152 FAD. |
Keyword | 3D-structure; Amino-acid biosynthesis; Complete proteome; FAD; Flavoprotein; Methionine biosynthesis; NAD; NADP; Oxidoreductase; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 296 AA |
Protein Sequence | MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS LKPKFVSVTY 60
GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR TIARDYWNNG IRHIVALRGD 120
LPPGSGKPEM YASDLVTLLK EVADFDISVA AYPEVHPEAK SAQADLLNLK RKVDAGANRA 180
ITQFFFDVES YLRFRDRCVS AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD 240
GLDDDAETRK LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL 296 |
Gene Ontology | GO:0005829; C:cytosol; IEA:InterPro. GO:0004489; F:methylenetetrahydrofolate reductase (NADPH) activity; IDA:EcoCyc. GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW. GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |