CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017597
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Saccharopine dehydrogenase-like oxidoreductase 
Protein Synonyms/Alias
  
Gene Name
 SCCPDH 
Gene Synonyms/Alias
 CGI-49 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58KLQRVLEKAALKLGRubiquitination[1]
144YHEKAADKGVYIIGSubiquitination[1]
220LRNVSNLKPVPLIGPubiquitination[1, 2, 3, 4, 5, 6, 7]
228PVPLIGPKLKRRWPIubiquitination[1, 7, 8]
242ISYCRELKGYSIPFMubiquitination[1, 3, 5, 7, 8, 9]
407TPGAAFSKTKLIDRLubiquitination[1, 3, 5, 8, 10]
409GAAFSKTKLIDRLNKubiquitination[1, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [10] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572
Functional Description
  
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 429 AA 
Protein Sequence
MATEQRPFHL VVFGASGFTG QFVTEEVARE QVDPERSSRL PWAVAGRSRE KLQRVLEKAA 60
LKLGRPTLSS EVGIIICDIA NPASLDEMAK QATVVLNCVG PYRFYGEPVI KACIENGASC 120
IDISGEPQFL ELMQLKYHEK AADKGVYIIG SSGFDSIPAD LGVIYTRNKM NGTLTAVESF 180
LTIHSGPEGL SIHDGTWKSA IYGFGDQSNL RKLRNVSNLK PVPLIGPKLK RRWPISYCRE 240
LKGYSIPFMG SDVSVVRRTQ RYLYENLEES PVQYAAYVTV GGITSVIKLM FAGLFFLFFV 300
RFGIGRQLLI KFPWFFSFGY FSKQGPTQKQ IDAASFTLTF FGQGYSQGTG TDKNKPNIKI 360
CTQVKGPEAG YVATPIAMVQ AAMTLLSDAS HLPKAGGVFT PGAAFSKTKL IDRLNKHGIE 420
FSVISSSEV 429 
Gene Ontology
 GO:0005811; C:lipid particle; IDA:UniProtKB.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW. 
Interpro
 IPR016040; NAD(P)-bd_dom.
 IPR005097; Saccharopine_DH/HSpermid_syn. 
Pfam
 PF03435; Saccharop_dh 
SMART
  
PROSITE
  
PRINTS