CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000516
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aspartate--tRNA ligase 
Protein Synonyms/Alias
 Aspartyl-tRNA synthetase; AspRS 
Gene Name
 aspS 
Gene Synonyms/Alias
 BSU27550 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
339GAGDYSRKDIDALGAacetylation[1]
496GSIRIFEKDIQEKMFacetylation[1]
587DELHLSIKKKVKN**acetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
  
Sequence Annotation
  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 592 AA 
Protein Sequence
MFGRTYYCGD ITEKAIGESV TLKGWVQKRR DLGGLIFIDL RDRTGIVQVV FNPDVSKEAL 60
AIAEGIRNEY VLDIQGKVVA REEGTVNPNL KTGAIEIHAD GVNVLNAAKT PPFAISDQAE 120
EVSEDVRLKH RYLDLRRPAM FQTMQLRHNV TKAVRSFLDE NGFLDIETPI LTGSTPEGAR 180
DYLVPSRVHE GEFYALPQSP QLFKQLLMVS GIERYYQIAR CFRDEDLRAD RQPEFTQIDI 240
EMSFMSQEDI MSLAEEMMAK VMRETKGEEL QLPLPRMTYD EAMNKYGSDK PDTRFDMLLT 300
DVSDIVKDTE FKVFSSAVAN GGVVKAINVK GGAGDYSRKD IDALGAFAAN YGAKGLAWVK 360
VEADGVKGPI AKFFDEEKQS KLIEALDAAE GDLLLFGADQ FEVVAASLGA LRLKLGKERG 420
LIDEKLFNFL WVIDWPLLEH DPEEGRFYAA HHPFTMPVRE DLELIETAPE DMKAQAYDLV 480
LNGYELGGGS IRIFEKDIQE KMFALLGFSP EEAAEQFGFL LEAFEYGAPP HGGIALGLDR 540
LVMLLAGRTN LRDTIAFPKT ASASCLMTEA PGEVSDAQLD ELHLSIKKKV KN 592 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004815; F:aspartate-tRNA ligase activity; IEA:HAMAP.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006422; P:aspartyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR004524; Asp-tRNA-ligase_IIb_bac/mt.
 IPR002312; Asp/Asn-tRNA-synth_IIb.
 IPR004115; GAD_dom.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 
Pfam
 PF02938; GAD
 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01042; TRNASYNTHASP.