CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011665
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 543 
Protein Synonyms/Alias
  
Gene Name
 ZNF543 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
72QELWMATKDLSQSSYubiquitination[1]
85SYPGDNTKPKTTEPTubiquitination[1, 2]
122NSQLGQSKDQDGPSEubiquitination[1]
166SDDGVCTKITQKQVSubiquitination[1]
170VCTKITQKQVSTEGDubiquitination[1]
225ERIHTQVKPYECTECubiquitination[1]
234YECTECGKTFSKSTHubiquitination[1]
309HRSHTGEKPFVCKECubiquitination[1]
314GEKPFVCKECGKAFRubiquitination[1]
337YIIHTGEKPYECIECubiquitination[1]
365QQIHTGVKPFECNECubiquitination[1]
449KRTHTGEKPYECKECubiquitination[2]
505QQIHTGEKPYECIQCubiquitination[1]
514YECIQCGKAFCRSANubiquitination[1]
533SIIHTGEKPYECSECubiquitination[1]
542YECSECGKAFNRGSSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 May be involved in transcriptional regulation. 
Sequence Annotation
 DOMAIN 9 80 KRAB.
 ZN_FING 199 221 C2H2-type 1.
 ZN_FING 227 249 C2H2-type 2.
 ZN_FING 255 277 C2H2-type 3.
 ZN_FING 283 305 C2H2-type 4.
 ZN_FING 311 333 C2H2-type 5.
 ZN_FING 339 361 C2H2-type 6.
 ZN_FING 367 389 C2H2-type 7.
 ZN_FING 395 417 C2H2-type 8.
 ZN_FING 423 445 C2H2-type 9.
 ZN_FING 451 473 C2H2-type 10.
 ZN_FING 479 501 C2H2-type 11.
 ZN_FING 507 529 C2H2-type 12.
 ZN_FING 535 557 C2H2-type 13.  
Keyword
 Complete proteome; DNA-binding; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 600 AA 
Protein Sequence
MAASAQVSVT FEDVAVTFTQ EEWGQLDAAQ RTLYQEVMLE TCGLLMSLGC PLFKPELIYQ 60
LDHRQELWMA TKDLSQSSYP GDNTKPKTTE PTFSHLALPE EVLLQEQLTQ GASKNSQLGQ 120
SKDQDGPSEM QEVHLKIGIG PQRGKLLEKM SSERDGLGSD DGVCTKITQK QVSTEGDLYE 180
CDSHGPVTDA LIREEKNSYK CEECGKVFKK NALLVQHERI HTQVKPYECT ECGKTFSKST 240
HLLQHLIIHT GEKPYKCMEC GKAFNRRSHL TRHQRIHSGE KPYKCSECGK AFTHRSTFVL 300
HHRSHTGEKP FVCKECGKAF RDRPGFIRHY IIHTGEKPYE CIECGKAFNR RSYLTWHQQI 360
HTGVKPFECN ECGKAFCESA DLIQHYIIHT GEKPYKCMEC GKAFNRRSHL KQHQRIHTGE 420
KPYECSECGK AFTHCSTFVL HKRTHTGEKP YECKECGKAF SDRADLIRHF SIHTGEKPYE 480
CVECGKAFNR SSHLTRHQQI HTGEKPYECI QCGKAFCRSA NLIRHSIIHT GEKPYECSEC 540
GKAFNRGSSL THHQRIHTGR NPTIVTDVGR PFMTAQTSVN IQELLLGKEF LNITTEENLW 600 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS