CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012756
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Thioredoxin reductase 1, cytoplasmic 
Protein Synonyms/Alias
 TR; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein; KM-102-derived reductase-like factor; Thioredoxin reductase TR1 
Gene Name
 TXNRD1 
Gene Synonyms/Alias
 GRIM12; KDRF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73SGGLAAAKEAAQYGKubiquitination[1]
133DSRNYGWKVEETVKHubiquitination[1]
139WKVEETVKHDWDRMIubiquitination[1]
168RVALREKKVVYENAYubiquitination[1]
290HMEEHGIKFIRQFVPubiquitination[1]
299IRQFVPIKVEQIEAGubiquitination[1]
343GRDACTRKIGLETVGubiquitination[1]
352GLETVGVKINEKTGKubiquitination[1]
359KINEKTGKIPVTDEEubiquitination[1]
383IGDILEDKVELTPVAubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. 
Sequence Annotation
 DOMAIN 56 156 Glutaredoxin.
 NP_BIND 192 209 FAD (By similarity).
 ACT_SITE 622 622 Proton acceptor (By similarity).
 MOD_RES 281 281 Phosphotyrosine.
 DISULFID 209 214 Redox-active (By similarity).
 CROSSLNK 647 648 Cysteinyl-selenocysteine (Cys-Sec) (By  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Electron transport; FAD; Flavoprotein; NADP; Nucleus; Oxidoreductase; Phosphoprotein; Polymorphism; Redox-active center; Reference proteome; Selenocysteine; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 649 AA 
Protein Sequence
MLSRLVLNSW AQAIIRPRPP KVLGLQVTTF SEAYQEGRLQ KLLKMNGPED LPKSYDYDLI 60
IIGGGSGGLA AAKEAAQYGK KVMVLDFVTP TPLGTRWGLG GTCVNVGCIP KKLMHQAALL 120
GQALQDSRNY GWKVEETVKH DWDRMIEAVQ NHIGSLNWGY RVALREKKVV YENAYGQFIG 180
PHRIKATNNK GKEKIYSAER FLIATGERPR YLGIPGDKEY CISSDDLFSL PYCPGKTLVV 240
GASYVALECA GFLAGIGLDV TVMVRSILLR GFDQDMANKI GEHMEEHGIK FIRQFVPIKV 300
EQIEAGTPGR LRVVAQSTNS EEIIEGEYNT VMLAIGRDAC TRKIGLETVG VKINEKTGKI 360
PVTDEEQTNV PYIYAIGDIL EDKVELTPVA IQAGRLLAQR LYAGSTVKCD YENVPTTVFT 420
PLEYGACGLS EEKAVEKFGE ENIEVYHSYF WPLEWTIPSR DNNKCYAKII CNTKDNERVV 480
GFHVLGPNAG EVTQGFAAAL KCGLTKKQLD STIGIHPVCA EVFTTLSVTK RSGASILQAG 540
CUG 543 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0045340; F:mercury ion binding; IEA:Compara.
 GO:0016174; F:NAD(P)H oxidase activity; IEA:Compara.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro.
 GO:0033797; F:selenate reductase activity; IEA:Compara.
 GO:0004791; F:thioredoxin-disulfide reductase activity; EXP:Reactome.
 GO:0042537; P:benzene-containing compound metabolic process; IEA:Compara.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0071280; P:cellular response to copper ion; IEA:Compara.
 GO:0071455; P:cellular response to hyperoxia; IEA:Compara.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0006749; P:glutathione metabolic process; IEA:Compara.
 GO:0070276; P:halogen metabolic process; IEA:Compara.
 GO:0042744; P:hydrogen peroxide catabolic process; IEA:Compara.
 GO:0001707; P:mesoderm formation; IEA:Compara.
 GO:0042191; P:methylmercury metabolic process; IEA:Compara.
 GO:0070995; P:NADPH oxidation; IEA:Compara.
 GO:0015949; P:nucleobase-containing small molecule interconversion; TAS:Reactome.
 GO:0001890; P:placenta development; IEA:Compara.
 GO:0010942; P:positive regulation of cell death; IEA:Compara.
 GO:0051262; P:protein tetramerization; IEA:Compara.
 GO:0048678; P:response to axon injury; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0010269; P:response to selenium ion; IEA:Compara.
 GO:0016259; P:selenocysteine metabolic process; IEA:Compara.
 GO:0007165; P:signal transduction; NAS:ProtInc. 
Interpro
 IPR016156; FAD/NAD-linked_Rdtase_dimer.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR002109; Glutaredoxin.
 IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR012999; Pyr_OxRdtase_I_AS.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom.
 IPR012336; Thioredoxin-like_fold.
 IPR006338; Thioredoxin/glutathione_Rdtase. 
Pfam
 PF00462; Glutaredoxin
 PF00070; Pyr_redox
 PF07992; Pyr_redox_2
 PF02852; Pyr_redox_dim 
SMART
  
PROSITE
 PS00195; GLUTAREDOXIN_1
 PS51354; GLUTAREDOXIN_2
 PS00076; PYRIDINE_REDOX_1 
PRINTS
 PR00368; FADPNR.