CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017773
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic peptide chain release factor GTP-binding subunit ERF3A 
Protein Synonyms/Alias
 Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog 
Gene Name
 Gspt1 
Gene Synonyms/Alias
 Erf3a 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
74RQLNVNAKPFVPNVHacetylation[1]
350LAKTAGVKHLIVLINacetylation[2]
391KVGFNPKKDIHFMPCacetylation[2]
391KVGFNPKKDIHFMPCubiquitination[3]
457MGTVVLGKLESGSICacetylation[2]
465LESGSICKGQQLVMMubiquitination[3]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (By similarity). 
Sequence Annotation
 NP_BIND 218 225 GTP (By similarity).
 NP_BIND 295 299 GTP (By similarity).
 NP_BIND 357 360 GTP (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; GTP-binding; Nonsense-mediated mRNA decay; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 636 AA 
Protein Sequence
MDPSSGGGGG GGGGGSSSSS DSAPDCWDQT DMEAPGPGPC GGGGSGSGSM AAVAEAQREN 60
LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAQPPLSP AGAAGGDHGA GSGAGGPSEP 120
VESSQDQSCE GSNSTVSMEL SEPVVENGET EMSPEESWEH KEEISEAEPG GGSSGDGRPP 180
EESTQEMMEE EEEIPKPKSA VAPPGAPKKE HVNVVFIGHV DAGKSTIGGQ IMYLTGMVDK 240
RTLEKYEREA KEKNRETWYL SWALDTNQEE RDKGKTVEVG RAYFETEKKH FTILDAPGHK 300
SFVPNMIGGA SQADLAVLVI SARKGEFETG FEKGGQTREH AMLAKTAGVK HLIVLINKMD 360
DPTVNWSNER YEECKEKLVP FLKKVGFNPK KDIHFMPCSG LTGANLKEQS DFCPWYIGLP 420
FIPYLDNLPN FNRSVDGPIR LPIVDKYKDM GTVVLGKLES GSICKGQQLV MMPNKHNVEV 480
LGILSDDVET DSVAPGENLK IRLKGIEEEE ILPGFILCDL NNLCHSGRTF DAQIVIIEHK 540
SIICPGYNAV LHIHTCIEEV EITALICLVD KKSGEKSKTR PRFVKQDQVC IARLRTAGTI 600
CLETFKDFPQ MGRFTLRDEG KTIAIGKVLK LVPEKD 636 
Gene Ontology
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0003747; F:translation release factor activity; ISS:UniProtKB.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
 GO:0006479; P:protein methylation; ISO:MGI. 
Interpro
 IPR009818; Ataxin-2_C.
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR004160; Transl_elong_EFTu/EF1A_C.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF03143; GTP_EFTU_D3
 PF07145; PAM2 
SMART
  
PROSITE
 PS00301; EFACTOR_GTP 
PRINTS
 PR00315; ELONGATNFCT.