CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011194
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative glucokinase-2 
Protein Synonyms/Alias
 Early meiotic induction protein 2; Glucose kinase 2; GLK-2 
Gene Name
 EMI2 
Gene Synonyms/Alias
 YDR516C; D9719.21 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
110TFDMQQLKSKIPEEYacetylation[1]
156KSTGENIKPLKMGFTubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Putative glucokinase involved in phosphorylation of aldohexoses and glucose uptake (By similarity). Involved in sporulation. Required for the full activation of the early meiotic inducer IME1. 
Sequence Annotation
 NP_BIND 487 492 ATP (By similarity).
 REGION 159 185 Glucose-binding (By similarity).
 BINDING 110 110 ATP (By similarity).  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Sporulation; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 500 AA 
Protein Sequence
MSFENLHKVN AEALEDAVVE ICSSLQVDAA KLDELTAYFI ECMEKGLNNT SVGEEKTVDK 60
GLPMIPTYVT SLPNGTERGV LLAADLGGTH FRVCSVTLNG DGTFDMQQLK SKIPEEYLND 120
KDVTSEELFS YLGRRTRAFV RKHHPELLKS TGENIKPLKM GFTFSYPVDQ TSLSSGTLIR 180
WTKSFKIEDT VGKDVVRLYQ EQLDIQGLSM INVVALTNDT VGTFLSHCYT SGSRPSSAGE 240
ISEPVIGCIF GTGTNGCYME DIENIKKLPD ELRTRLLHEG KTQMCINIEW GSFDNELKHL 300
SATKYDIDID QKFSPNPGYH LFEKRISGMY LGELLRNILV DLHARGLILG QYRNYDQLPH 360
RLKTPFQLCS EVLSRIEIDD STNLRETELS FLQSLRLPTT FEERKAIQNL VRSITRRSAY 420
LAAVPIAAIL IKTNALNKRY HGEVEIGFDG YVIEYYPGFR SMLRHALALS PIGTEGERKI 480
HLRLAKDGSG VGAALCALVA 500 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004340; F:glucokinase activity; IEA:EC.
 GO:0030437; P:ascospore formation; IMP:SGD.
 GO:0051156; P:glucose 6-phosphate metabolic process; IEA:GOC.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:SGD. 
Interpro
 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 
Pfam
 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 
SMART
  
PROSITE
 PS00378; HEXOKINASES 
PRINTS
 PR00475; HEXOKINASE.