UniProt Accession

 SET_RAT; Q63945; Q7TPA3 

Genbank Protein ID

 S68589; S68987; AY325137 

Genbank Nucleotide ID

 AAC60681.1; AAC60682.1; AAP92538.1 

Protein Name

 Protein SET 

Protein Synonyms/Alias

 Liver regeneration-related protein LRRGR00002; Phosphatase 2A inhibitor I2PP2A; I-2PP2A; Template-activating factor I; TAF-I 

Gene Name

 Set 

Gene Synonyms/Alias

 Ab1-115 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
23	PRPVAAPKLEDKSAS	acetylation	[1]
27	AAPKLEDKSASPGLP	acetylation	[1]
38	PGLPKGEKEQQEAIE	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T- lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher (By similarity). 

Sequence Annotation

 REGION 31 77 Dimerization (By similarity).
 REGION 78 224 Earmuff domain (By similarity).
 MOD_RES 2 2 N,N,N-trimethylalanine (By similarity).
 MOD_RES 7 7 Phosphoserine (By similarity).
 MOD_RES 131 131 N6-acetyllysine (By similarity).
 MOD_RES 145 145 Phosphotyrosine (By similarity).
 MOD_RES 149 149 N6-acetyllysine (By similarity).
 MOD_RES 171 171 N6-acetyllysine (By similarity).
 CROSSLNK 153 153 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Alternative splicing; Chaperone; Complete proteome; Cytoplasm; DNA-binding; Endoplasmic reticulum; Isopeptide bond; Methylation; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 289 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0006334; P:nucleosome assembly; IEA:InterPro. 

Interpro

 IPR002164; NAP_family. 

Pfam

 PF00956; NAP 

SMART

  

PROSITE

  

PRINTS