| Tag | Content |
|---|
| CPLM ID | CPLM-002764 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | PTS system N-acetylglucosamine-specific EIICBA component |
Protein Synonyms/Alias | EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component |
Gene Name | nagE |
Gene Synonyms/Alias | pstN; b0679; JW0665 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 130 | FLSFFGGKRFVPIAT | acetylation | [1, 2] | | 372 | KTPGREDKEDEIVTE | acetylation | [2] | | 536 | VAVKPTDKIVVSPAA | acetylation | [2] | | 647 | QTPLYEIKK****** | acetylation | [2] |
|
Reference | [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli. Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z. J Proteome Res. 2013 Feb 1;12(2):844-51. [ PMID: 23294111] [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N-acetylglucosamine transport. |
Sequence Annotation | DOMAIN 1 371 PTS EIIC type-1.
DOMAIN 390 472 PTS EIIB type-1.
DOMAIN 517 621 PTS EIIA type-1.
ACT_SITE 412 412 Phosphocysteine intermediate; for EIIB
ACT_SITE 569 569 Tele-phosphohistidine intermediate; for |
Keyword | Cell inner membrane; Cell membrane; Complete proteome; Kinase; Membrane; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transmembrane; Transmembrane helix; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 648 AA |
Protein Sequence | MNILGFFQRL GRALQLPIAV LPVAALLLRF GQPDLLNVAF IAQAGGAIFD NLALIFAIGV 60
ASSWSKDSAG AAALAGAVGY FVLTKAMVTI NPEINMGVLA GIITGLVGGA AYNRWSDIKL 120
PDFLSFFGGK RFVPIATGFF CLVLAAIFGY VWPPVQHAIH AGGEWIVSAG ALGSGIFGFI 180
NRLLIPTGLH QVLNTIAWFQ IGEFTNAAGT VFHGDINRFY AGDGTAGMFM SGFFPIMMFG 240
LPGAALAMYF AAPKERRPMV GGMLLSVAVT AFLTGVTEPL EFLFMFLAPL LYLLHALLTG 300
ISLFVATLLG IHAGFSFSAG AIDYALMYNL PAASQNVWML LVMGVIFFAI YFVVFSLVIR 360
MFNLKTPGRE DKEDEIVTEE ANSNTEEGLT QLATNYIAAV GGTDNLKAID ACITRLRLTV 420
ADSARVNDTM CKRLGASGVV KLNKQTIQVI VGAKAESIGD AMKKVVARGP VAAASAEATP 480
ATAAPVAKPQ AVPNAVSIAE LVSPITGDVV ALDQVPDEAF ASKAVGDGVA VKPTDKIVVS 540
PAAGTIVKIF NTNHAFCLET EKGAEIVVHM GIDTVALEGK GFKRLVEEGA QVSAGQPILE 600
MDLDYLNANA RSMISPVVCS NIDDFSGLII KAQGHIVAGQ TPLYEIKK 648 |
Gene Ontology | GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0016020; C:membrane; NAS:UniProtKB. GO:0019866; C:organelle inner membrane; IEA:InterPro. GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. GO:0016301; F:kinase activity; IEA:UniProtKB-KW. GO:0015572; F:N-acetylglucosamine transmembrane transporter activity; IEA:InterPro. GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:EC. GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro. GO:0016740; F:transferase activity; NAS:UniProtKB. GO:0005215; F:transporter activity; NAS:UniProtKB. GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW. GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |