CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015791
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RAS guanyl-releasing protein 2 
Protein Synonyms/Alias
 Calcium and DAG-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25-like protein; hCDC25L; F25B3.3 kinase-like protein 
Gene Name
 RASGRP2 
Gene Synonyms/Alias
 CDC25L; MCG7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71AGTLDLDKGCTVEELubiquitination[1, 2]
91EAFDDSGKVRDPQLVubiquitination[2]
136NSNSLQVKTCHLVRYubiquitination[2]
190IDSVPTYKWKRQVTQubiquitination[3]
192SVPTYKWKRQVTQRNubiquitination[2]
321HSSISRLKETHSHVSubiquitination[2]
586ALILGIYKQGLKCRAubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. 
Sequence Annotation
 DOMAIN 4 126 N-terminal Ras-GEF.
 DOMAIN 154 387 Ras-GEF.
 DOMAIN 426 461 EF-hand 1.
 DOMAIN 455 490 EF-hand 2.
 ZN_FING 498 548 Phorbol-ester/DAG-type.
 MOD_RES 116 116 Phosphoserine (By similarity).
 MOD_RES 117 117 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Calcium; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Synapse; Synaptosome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 609 AA 
Protein Sequence
MGTQRLCGRG TQGWPGSSEQ HVQEATSSAG LHSGVDELGV RSEPGGRLPE RSLGPAHPAP 60
AAMAGTLDLD KGCTVEELLR GCIEAFDDSG KVRDPQLVRM FLMMHPWYIP SSQLAAKLLH 120
IYQQSRKDNS NSLQVKTCHL VRYWISAFPA EFDLNPELAE QIKELKALLD QEGNRRHSSL 180
IDIDSVPTYK WKRQVTQRNP VGQKKRKMSL LFDHLEPMEL AEHLTYLEYR SFCKILFQDY 240
HSFVTHGCTV DNPVLERFIS LFNSVSQWVQ LMILSKPTAP QRALVITHFV HVAEKLLQLQ 300
NFNTLMAVVG GLSHSSISRL KETHSHVSPE TIKLWEGLTE LVTATGNYGN YRRRLAACVG 360
FRFPILGVHL KDLVALQLAL PDWLDPARTR LNGAKMKQLF SILEELAMVT SLRPPVQANP 420
DLLSLLTVSL DQYQTEDELY QLSLQREPRS KSSPTSPTSC TPPPRPPVLE EWTSAAKPKL 480
DQALVVEHIE KMVESVFRNF DVDGDGHISQ EEFQIIRGNF PYLSAFGDLD QNQDGCISRE 540
EMVSYFLRSS SVLGGRMGFV HNFQESNSLR PVACRHCKAL ILGIYKQGLK CRACGVNCHK 600
QCKDRLSVEC RRRAQSVSLE GSAPSPSPMH SHHHRAFSFS LPRPGRRGSR PPEIREEEVQ 660
TVEDGVFDIH L 671 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0043005; C:neuron projection; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0045202; C:synapse; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0019992; F:diacylglycerol binding; NAS:UniProtKB.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; TAS:UniProtKB.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0007265; P:Ras protein signal transduction; NAS:UniProtKB.
 GO:0001558; P:regulation of cell growth; NAS:UniProtKB.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25. 
Pfam
 PF00130; C1_1
 PF13202; EF_hand_3
 PF00617; RasGEF
 PF00618; RasGEF_N 
SMART
 SM00109; C1
 SM00054; EFh
 SM00147; RasGEF
 SM00229; RasGEFN 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00720; RASGEF
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS