| Tag | Content |
|---|
| CPLM ID | CPLM-023856 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Cysteine protease ATG4B |
Protein Synonyms/Alias | AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B |
Gene Name | ATG4B |
Gene Synonyms/Alias | APG4B; AUTL1; KIAA0943 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 207 | LNAFIDRKDSYYSIH | ubiquitination | [1] | | 242 | TVAQVLKKLAVFDTW | ubiquitination | [1] | | 425 | NDWCQQVKKLSLLGG | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Cysteine protease required for autophagy, which cleaves the C-terminal part of MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Also mediates the lipid deconjugation required for target recycling. |
Sequence Annotation | ACT_SITE 74 74 Nucleophile.
ACT_SITE 278 278 Potential.
ACT_SITE 280 280
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 34 34 Phosphoserine (By similarity).
MOD_RES 383 383 Phosphoserine. |
Keyword | 3D-structure; Acetylation; Alternative splicing; Autophagy; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Phosphoprotein; Polymorphism; Protease; Protein transport; Reference proteome; Thiol protease; Transport; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 393 AA |
Protein Sequence | MAHSVPSDSR TSRRPTTRPH AARGAPRGSR RPGRTPKWRL PRISARAPYR LRRLRRHTYW 60
PPRRPVAASR CWPVGATPLG SVGGRTGKMD AATLTYDTLR FAEFEDFPET SEPVWILGRK 120
YSIFTEKDEI LSDVASRLWF TYRKNFPAIG GTGPTSDTGW GCMLRCGQMI FAQALVCRHL 180
GRDWRWTQRK RQPDSYFSVL NAFIDRKDSY YSIHQIAQMG VGEGKSIGQW YGPNTVAQVL 240
KKLAVFDTWS SLAVHIAMDN TVVMEEIRRL CRTSVPCAGA TAFPADSDRH CNGFPAGAEV 300
TNRPSPWRPL VLLIPLRLGL TDINEAYVET LKHCFMMPQS LGVIGGKPNS AHYFIGYVGE 360
ELIYLDPHTT QPAVEPTDGC FIPDESFHCQ HPPCRMSIAE LDPSIAVGFF CKTEDDFNDW 420
CQQVKKLSLL GGALPMFELV ELQPSHLACP DVLNLSLGES CQVQILLM 468 |
Gene Ontology | GO:0005737; C:cytoplasm; ISS:UniProtKB. GO:0008234; F:cysteine-type peptidase activity; IDA:UniProtKB. GO:0004175; F:endopeptidase activity; IDA:BHF-UCL. GO:0000045; P:autophagic vacuole assembly; IGI:UniProtKB. GO:0010508; P:positive regulation of autophagy; IDA:BHF-UCL. GO:0015031; P:protein transport; IEA:UniProtKB-KW. GO:0006508; P:proteolysis; IDA:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |