CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012795
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chromatin-remodeling complex ATPase chain Iswi 
Protein Synonyms/Alias
 CHRAC 140 kDa subunit; Nucleosome-remodeling factor 140 kDa subunit; NURF-140; Protein imitation swi 
Gene Name
 Iswi 
Gene Synonyms/Alias
 CG8625 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
337TRLHAVLKPFLLRRLacetylation[1]
388DVVNGAGKVEKMRLQacetylation[1]
753FRKTVGYKVPKNTELacetylation[2]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702]
 [2] Site-specific acetylation of ISWI by GCN5.
 Ferreira R, Eberharter A, Bonaldi T, Chioda M, Imhof A, Becker PB.
 BMC Mol Biol. 2007 Aug 30;8:73. [PMID: 17760996] 
Functional Description
 Energy-transducing component of the chromatin-remodeling complexes NURF (nucleosome-remodeling factor), ACF (ATP-utilizing chromatin assembly and remodeling factor), and CHRAC (chromatin accessibility complex). NURF catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. It is required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis. 
Sequence Annotation
 DOMAIN 140 305 Helicase ATP-binding.
 DOMAIN 435 586 Helicase C-terminal.
 DOMAIN 795 847 SANT 1.
 DOMAIN 898 962 SANT 2.
 NP_BIND 153 160 ATP (Potential).
 MOTIF 256 259 DEAH box.
 MOD_RES 67 67 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Chromatin regulator; Complete proteome; Direct protein sequencing; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1027 AA 
Protein Sequence
MSKTDTAAVE ATEENSNETT SDAATSSSGE KEAEFDNKIE ADRSRRFDFL LKQTEIFTHF 60
MTNSAKSPTK PKGRPKKIKD KDKEKDVADH RHRKTEQEED EELLAEDSAT KEIFRFDASP 120
AYIKSGEMRD YQIRGLNWMI SLYENGINGI LADEMGLGKT LQTISLLGYL KHFKNQAGPH 180
IVIVPKSTLQ NWVNEFKKWC PSLRAVCLIG DQDTRNTFIR DVLMPGEWDV CVTSYEMCIR 240
EKSVFKKFNW RYLVIDEAHR IKNEKSKLSE ILREFKTANR LLITGTPLQN NLHELWALLN 300
FLLPDVFNSS EDFDEWFNTN TCLGDDALIT RLHAVLKPFL LRRLKAEVEK RLKPKKEMKI 360
FVGLSKMQRD WYTKVLLKDI DVVNGAGKVE KMRLQNILMQ LRKCTNHPYL FDGAEPGPPY 420
TTDTHLVYNS GKMAILDKLL PKLQEQGSRV LIFSQMTRML DILEDYCHWR NYNYCRLDGQ 480
TPHEDRNRQI QEFNMDNSAK FLFMLSTRAG GLGINLATAD VVIIYDSDWN PQMDLQAMDR 540
AHRIGQKKQV RVFRLITEST VEEKIVERAE VKLRLDKMVI QGGRLVDNRS NQLNKDEMLN 600
IIRFGANQVF SSKETDITDE DIDVILERGE AKTAEQKAAL DSLGESSLRT FTMDTNGEAG 660
TSSVYQFEGE DWREKQKLNA LGNWIEPPKR ERKANYAVDA YFREALRVSE PKAPKAPRPP 720
KQPIVQDFQF FPPRLFELLD QEIYYFRKTV GYKVPKNTEL GSDATKVQRE EQRKIDEAEP 780
LTEEEIQEKE NLLSQGFTAW TKRDFNQFIK ANEKYGRDDI DNIAKDVEGK TPEEVIEYNA 840
VFWERCTELQ DIERIMGQIE RGEGKIQRRL SIKKALDQKM SRYRAPFHQL RLQYGNNKGK 900
NYTEIEDRFL VCMLHKLGFD KENVYEELRA AIRASPQFRF DWFIKSRTAL ELQRRCNTLI 960
TLIERENIEL EEKERAEKKK KAPKGSVSAG SGSASSNTPA PAPQPKASQK RKSEVVATSS 1020
NSKKKKK 1027 
Gene Ontology
 GO:0016590; C:ACF complex; IDA:FlyBase.
 GO:0008623; C:CHRAC; IDA:FlyBase.
 GO:0016589; C:NURF complex; IDA:FlyBase.
 GO:0005700; C:polytene chromosome; IDA:FlyBase.
 GO:0031213; C:RSF complex; IPI:FlyBase.
 GO:0005667; C:transcription factor complex; IPI:FlyBase.
 GO:0005524; F:ATP binding; ISS:FlyBase.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003678; F:DNA helicase activity; TAS:FlyBase.
 GO:0008094; F:DNA-dependent ATPase activity; IDA:FlyBase.
 GO:0031491; F:nucleosome binding; IEA:InterPro.
 GO:0070615; F:nucleosome-dependent ATPase activity; IDA:FlyBase.
 GO:0043044; P:ATP-dependent chromatin remodeling; IEA:InterPro.
 GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
 GO:0035076; P:ecdysone receptor-mediated signaling pathway; IGI:FlyBase.
 GO:0007517; P:muscle organ development; IMP:FlyBase.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IMP:FlyBase.
 GO:0035063; P:nuclear speck organization; IMP:FlyBase.
 GO:0006334; P:nucleosome assembly; IDA:FlyBase.
 GO:0042766; P:nucleosome mobilization; IDA:FlyBase.
 GO:0016584; P:nucleosome positioning; IDA:FlyBase.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:FlyBase.
 GO:0042752; P:regulation of circadian rhythm; IMP:FlyBase.
 GO:0006351; P:transcription, DNA-dependent; IDA:FlyBase. 
Interpro
 IPR020838; DBINO.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR009057; Homeodomain-like.
 IPR015194; ISWI_HAND-dom.
 IPR027417; P-loop_NTPase.
 IPR001005; SANT/Myb.
 IPR017884; SANT_dom.
 IPR015195; SLIDE.
 IPR000330; SNF2_N. 
Pfam
 PF13892; DBINO
 PF09110; HAND
 PF00271; Helicase_C
 PF09111; SLIDE
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc
 SM00717; SANT 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51293; SANT 
PRINTS