UniProt Accession

 SYYC_RAT; Q4KM49 

Genbank Protein ID

 BC098795 

Genbank Nucleotide ID

 AAH98795.1 

Protein Name

 Tyrosine--tRNA ligase, cytoplasmic 

Protein Synonyms/Alias

 Tyrosyl-tRNA synthetase; TyrRS; Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed 

Gene Name

 Yars 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
26	QEVLGEEKLKEILKE	acetylation	[1]
146	LVTQHDAKKAGAEVV	acetylation	[1]
190	FGGIDQRKIFTFAEK	acetylation	[1]
197	KIFTFAEKYLPTLGY	acetylation	[1]
206	LPTLGYSKRVHLMNP	acetylation	[1]
319	SVEVALNKLLDPIRE	acetylation	[1]
482	GQPDEELKPKKKVFE	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity). 

Sequence Annotation

 DOMAIN 364 468 tRNA-binding.
 MOTIF 44 52 "HIGH" region (By similarity).
 MOTIF 222 226 "KMSKS" region (By similarity).
 BINDING 39 39 Tyrosine (By similarity).
 BINDING 166 166 Tyrosine (By similarity).
 BINDING 170 170 Tyrosine (By similarity).
 BINDING 173 173 Tyrosine (By similarity).
 BINDING 188 188 Tyrosine (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 N-acetylglycine; in Tyrosine--tRNA
 MOD_RES 197 197 N6-acetyllysine (By similarity).
 MOD_RES 206 206 N6-acetyllysine (By similarity).
 MOD_RES 474 474 N6-acetyllysine (By similarity).
 MOD_RES 482 482 N6-acetyllysine (By similarity).
 MOD_RES 490 490 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 528 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
 GO:0004831; F:tyrosine-tRNA ligase activity; IEA:EC.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:InterPro. 

Interpro

 IPR002305; aa-tRNA-synth_Ic.
 IPR012340; NA-bd_OB-fold.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002547; tRNA-bd_dom.
 IPR002307; Tyr-tRNA-ligase.
 IPR023617; Tyr-tRNA-ligase_arc/euk-type. 

Pfam

 PF00579; tRNA-synt_1b
 PF01588; tRNA_bind 

SMART

  

PROSITE

 PS50886; TRBD 

PRINTS

 PR01040; TRNASYNTHTYR.