CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-025018
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleoside diphosphate kinase 
Protein Synonyms/Alias
  
Gene Name
 NME1-NME2 
Gene Synonyms/Alias
 NME2; hCG_2001850 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37ERTFIAIKPDGVQRGubiquitination[1, 2, 3, 4]
51GLVGEIIKRFEQKGFubiquitination[1, 3, 4]
56IIKRFEQKGFRLVGLubiquitination[4]
64GFRLVGLKFMQASEDubiquitination[1]
74QASEDLLKEHYVDLKubiquitination[1, 4]
81KEHYVDLKDRPFFAGubiquitination[1, 3, 4]
110WEGLNVVKTGRVMLGubiquitination[1]
125ETNPADSKPGTIRGDubiquitination[1, 2, 3, 4]
179GFRLVAMKFLRASEEubiquitination[3, 4, 5]
189RASEEHLKQHYIDLKubiquitination[3, 4, 5]
196KQHYIDLKDRPFFPGubiquitination[1, 3, 4, 5]
225WEGLNVVKTGRVMLGubiquitination[4]
264IHGSDSVKSAEKEISacetylation[6]
264IHGSDSVKSAEKEISubiquitination[1, 3, 4, 5, 7]
268DSVKSAEKEISLWFKubiquitination[1, 4, 5]
275KEISLWFKPEELVDYubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [7] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 292 AA 
Protein Sequence
MVLLSTLGIV FQGEGPPISS CDTGTMANCE RTFIAIKPDG VQRGLVGEII KRFEQKGFRL 60
VGLKFMQASE DLLKEHYVDL KDRPFFAGLV KYMHSGPVVA MVWEGLNVVK TGRVMLGETN 120
PADSKPGTIR GDFCIQVGRT MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF 180
LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK 240
PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE 292 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004550; F:nucleoside diphosphate kinase activity; IEA:EC.
 GO:0006241; P:CTP biosynthetic process; IEA:InterPro.
 GO:0006183; P:GTP biosynthetic process; IEA:InterPro.
 GO:0006228; P:UTP biosynthetic process; IEA:InterPro. 
Interpro
 IPR001564; Nucleoside_diP_kinase.
 IPR023005; Nucleoside_diP_kinase_AS. 
Pfam
 PF00334; NDK 
SMART
 SM00562; NDK 
PROSITE
 PS00469; NDP_KINASES 
PRINTS
 PR01243; NUCDPKINASE.