UniProt Accession

 CHMP3_MOUSE; Q9CQ10; Q9D2Z2; Q9D7A5 

Genbank Protein ID

 AK009414; AK014818; AK016677; AK018611; AK083562; BC049964 

Genbank Nucleotide ID

 BAB26273.1; BAB29566.1; BAB30375.1; BAB31306.1; BAC38951.1; AAH49964.1 

Protein Name

 Charged multivesicular body protein 3 

Protein Synonyms/Alias

 Chromatin-modifying protein 3; Vacuolar protein sorting-associated protein 24 

Gene Name

 Chmp3 

Gene Synonyms/Alias

 Vps24 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
112	QKSTEVMKAMQSLVK	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor (By similarity). 

Sequence Annotation

 REGION 2 113 Intramolecular interaction with C-
 REGION 59 64 Important for autoinhibitory function (By
 REGION 151 224 Interaction with VPS4A (By similarity).
 REGION 151 222 Intramolecular interaction with N-
 REGION 168 169 Important for autoinhibitory function (By
 REGION 196 224 Interaction with STAMBP (By similarity).
 REGION 205 209 Interaction with STAMBP (By similarity).
 REGION 223 224 Interaction with STAMBP (By similarity).
 MOTIF 201 213 MIT-interacting motif (By similarity).
 BINDING 218 218 STAMBP (By similarity).
 MOD_RES 200 200 Phosphoserine.
 LIPID 2 2 N-myristoyl glycine (Potential).
 CROSSLNK 179 179 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Endosome; Isopeptide bond; Lipoprotein; Membrane; Myristate; Phosphoprotein; Protein transport; Reference proteome; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 224 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005769; C:early endosome; IEA:Compara.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IEA:Compara.
 GO:0000910; P:cytokinesis; IEA:Compara.
 GO:0008333; P:endosome to lysosome transport; IEA:Compara.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0051036; P:regulation of endosome size; IEA:Compara. 

Interpro

 IPR005024; Snf7. 

Pfam

 PF03357; Snf7 

SMART

  

PROSITE

  

PRINTS