CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023075
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein fem-1 homolog B 
Protein Synonyms/Alias
 FEM1b; FEM1-beta; Fem-1-like death receptor-binding protein alpha; Fem-1-like in apoptotic pathway protein alpha; F1A-alpha 
Gene Name
 FEM1B 
Gene Synonyms/Alias
 F1AA; KIAA0396 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
60AARNGHAKVVRLLLEubiquitination[1]
231KVAAESCKADVVELLubiquitination[2]
469EDQCKINKQIYNLIHubiquitination[3, 4, 5]
579QNKTPLDKSTTGVSEubiquitination[2]
594ILLKTQMKMSLKCLAubiquitination[2]
598TQMKMSLKCLAARAVubiquitination[2, 4]
Reference
 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1. 
Sequence Annotation
 REPEAT 45 74 ANK 1.
 REPEAT 87 116 ANK 2.
 REPEAT 120 149 ANK 3.
 REPEAT 153 182 ANK 4.
 REPEAT 186 215 ANK 5.
 REPEAT 218 248 ANK 6.
 REPEAT 344 377 TPR.
 REPEAT 483 527 ANK 7.
 REPEAT 531 568 ANK 8.  
Keyword
 ANK repeat; Apoptosis; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Repeat; TPR repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 627 AA 
Protein Sequence
MEGLAGYVYK AASEGKVLTL AALLLNRSES DIRYLLGYVS QQGGQRSTPL IIAARNGHAK 60
VVRLLLEHYR VQTQQTGTVR FDGYVIDGAT ALWCAAGAGH FEVVKLLVSH GANVNHTTVT 120
NSTPLRAACF DGRLDIVKYL VENNANISIA NKYDNTCLMI AAYKGHTDVV RYLLEQRADP 180
NAKAHCGATA LHFAAEAGHI DIVKELIKWR AAIVVNGHGM TPLKVAAESC KADVVELLLS 240
HADCDRRSRI EALELLGASF ANDRENYDII KTYHYLYLAM LERFQDGDNI LEKEVLPPIH 300
AYGNRTECRN PQELESIRQD RDALHMEGLI VRERILGADN IDVSHPIIYR GAVYADNMEF 360
EQCIKLWLHA LHLRQKGNRN THKDLLRFAQ VFSQMIHLNE TVKAPDIECV LRCSVLEIEQ 420
SMNRVKNISD ADVHNAMDNY ECNLYTFLYL VCISTKTQCS EEDQCKINKQ IYNLIHLDPR 480
TREGFTLLHL AVNSNTPVDD FHTNDVCSFP NALVTKLLLD CGAEVNAVDN EGNSALHIIV 540
QYNRPISDFL TLHSIIISLV EAGAHTDMTN KQNKTPLDKS TTGVSEILLK TQMKMSLKCL 600
AARAVRANDI NYQDQIPRTL EEFVGFH 627 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005123; F:death receptor binding; IMP:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IMP:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0060442; P:branching involved in prostate gland morphogenesis; IEA:Compara.
 GO:0060743; P:epithelial cell maturation involved in prostate gland development; IEA:Compara.
 GO:0006917; P:induction of apoptosis; NAS:UniProtKB.
 GO:2000001; P:regulation of DNA damage checkpoint; IMP:UniProtKB.
 GO:1902041; P:regulation of extrinsic apoptotic signaling pathway via death domain receptors; IMP:UniProtKB.
 GO:0051438; P:regulation of ubiquitin-protein ligase activity; IMP:UniProtKB. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom. 
Pfam
 PF00023; Ank 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS
 PR01415; ANKYRIN.