CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020324
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase PINK1, mitochondrial 
Protein Synonyms/Alias
 BRPK; PTEN-induced putative kinase protein 1 
Gene Name
 PINK1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
186PQNLEVTKSTGLLPGubiquitination[1, 2]
319RTLFLVMKNYPCTLRubiquitination[1, 2, 3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. 
Sequence Annotation
 DOMAIN 156 511 Protein kinase.
 NP_BIND 162 170 ATP (By similarity).
 ACT_SITE 362 362 Proton acceptor (By similarity).
 BINDING 186 186 ATP (By similarity).
 MOD_RES 228 228 Phosphoserine; by autocatalysis.
 MOD_RES 402 402 Phosphoserine; by autocatalysis.  
Keyword
 Alternative splicing; ATP-binding; Autophagy; Complete proteome; Cytoplasm; Disease mutation; Kinase; Magnesium; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Neurodegeneration; Nucleotide-binding; Parkinsonism; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Transit peptide; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 581 AA 
Protein Sequence
MAVRQALGRG LQLGRALLLR FTGKPGRAYG LGRPGPAAGC VRGERPGWAA GPGAEPRRVG 60
LGLPNRLRFF RQSVAGLAAR LQRQFVVRAW GCAGPCGRAV FLAFGLGLGL IEEKQAESRR 120
AVSACQEIQA IFTQKSKPGP DPLDTRRLQG FRLEEYLIGQ SIGKGCSAAV YEATMPTLPQ 180
NLEVTKSTGL LPGRGPGTSA PGEGQERAPG APAFPLAIKM MWNISAGSSS EAILNTMSQE 240
LVPASRVALA GEYGAVTYRK SKRGPKQLAP HPNIIRVLRA FTSSVPLLPG ALVDYPDVLP 300
SRLHPEGLGH GRTLFLVMKN YPCTLRQYLC VNTPSPRLAA MMLLQLLEGV DHLVQQGIAH 360
RDLKSDNILV ELDPDGCPWL VIADFGCCLA DESIGLQLPF SSWYVDRGGN GCLMAPEVST 420
ARPGPRAVID YSKADAWAVG AIAYEIFGLV NPFYGQGKAH LESRSYQEAQ LPALPESVPP 480
DVRQLVRALL QREASKRPSA RVAANVLHLS LWGEHILALK NLKLDKMVGW LLQQSAATLL 540
ANRLTEKCCV ETKMKMLFLA NLECETLCQA ALLLCSWRAA L 581 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0010857; F:calcium-dependent protein kinase activity; IDA:BHF-UCL.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0097237; P:cellular response to toxic substance; IEA:Compara.
 GO:0007243; P:intracellular protein kinase cascade; IDA:UniProtKB.
 GO:0000422; P:mitochondrion degradation; IMP:UniProtKB.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:BHF-UCL.
 GO:0033603; P:positive regulation of dopamine secretion; IEA:Compara.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IDA:BHF-UCL.
 GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; IMP:BHF-UCL.
 GO:0032226; P:positive regulation of synaptic transmission, dopaminergic; IEA:Compara.
 GO:0043254; P:regulation of protein complex assembly; IDA:BHF-UCL.
 GO:0031396; P:regulation of protein ubiquitination; IDA:BHF-UCL.
 GO:0006950; P:response to stress; IDA:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS