CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000492
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitogen-activated protein kinase kinase kinase 3 
Protein Synonyms/Alias
 Arabidopsis NPK1-related protein kinase 3 
Gene Name
 ANP3 
Gene Synonyms/Alias
 At3g06030; F24F17.1; F2O10.1 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
108IAPSSASKEKTQGHIubiquitination[1]
110PSSASKEKTQGHIREubiquitination[1]
Reference
 [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants.
 Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K.
 Mol Cell Proteomics. 2007 Apr;6(4):601-10. [PMID: 17272265] 
Functional Description
 Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1. 
Sequence Annotation
 DOMAIN 68 330 Protein kinase.
 NP_BIND 74 82 ATP (By similarity).
 ACT_SITE 196 196 Proton acceptor (By similarity).
 BINDING 97 97 ATP (By similarity).
 CROSSLNK 108 108 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 110 110 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Isopeptide bond; Kinase; Microtubule; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 651 AA 
Protein Sequence
MQDILGSVRR SLVFRSSLAG DDGTSGGGLS GFVGKINSSI RSSRIGLFSK PPPGLPAPRK 60
EEAPSIRWRK GELIGCGAFG RVYMGMNLDS GELLAIKQVL IAPSSASKEK TQGHIRELEE 120
EVQLLKNLSH PNIVRYLGTV RESDSLNILM EFVPGGSISS LLEKFGSFPE PVIIMYTKQL 180
LLGLEYLHNN GIMHRDIKGA NILVDNKGCI RLADFGASKK VVELATVNGA KSMKGTPYWM 240
APEVILQTGH SFSADIWSVG CTVIEMATGK PPWSEQYQQF AAVLHIGRTK AHPPIPEDLS 300
PEAKDFLMKC LHKEPSLRLS ATELLQHPFV TGKRQEPYPA YRNSLTECGN PITTQGMNVR 360
SSINSLIRRS TCSGLKDVCE LGSLRSSIIY PQKSNNSGFG WRDGDSDDLC QTDMDDLCNI 420
ESVRNNVLSQ STDLNKSFNP MCDSTDNWSC KFDESPKVMK SKSNLLSYQA SQLQTGVPCD 480
EETSLTFAGG SSVAEDDYKG TELKIKSFLD EKAQDLKRLQ TPLLEEFHNA MNPGIPQGAL 540
GDTNIYNLPN LPSISKTPKR LPSRRLSAIS DAMPSPLKSS KRTLNTSRVM QSGTEPTQVN 600
ESTKKGVNNS RCFSEIRRKW EEELYEELER HRENLRHAGA GGKTPLSGHK G 651 
Gene Ontology
 GO:0048046; C:apoplast; IDA:TAIR.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004709; F:MAP kinase kinase kinase activity; IEA:EC.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:TAIR.
 GO:0000186; P:activation of MAPKK activity; IEA:GOC.
 GO:0043622; P:cortical microtubule organization; IMP:UniProtKB.
 GO:0000165; P:MAPK cascade; IEA:GOC.
 GO:0046777; P:protein autophosphorylation; IDA:TAIR. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS