CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021355
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinetochore-associated protein DSN1 homolog 
Protein Synonyms/Alias
  
Gene Name
 DSN1 
Gene Synonyms/Alias
 C20orf172; MIS13 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
76HQERLQSKSLHLSPQubiquitination[1]
108MKETNRRKSLHPIHQubiquitination[1]
132SVDLAESKRLGCLLLubiquitination[1]
157EPFLRDTKGFSLESFubiquitination[1]
167SLESFRAKASSLSEEubiquitination[1]
176SSLSEELKHFADGLEubiquitination[1, 2]
212EASVAEMKEYITKFSubiquitination[1, 2]
217EMKEYITKFSLERQTubiquitination[1, 3, 4]
237LHYQQEAKEILSRGSubiquitination[1]
248SRGSTEAKITEVKVEubiquitination[1, 2]
253EAKITEVKVEPMTYLubiquitination[1]
271QNEVLNTKPDYQKILubiquitination[1, 2]
276NTKPDYQKILQNQSKubiquitination[2]
322KVSVQLGKRSMQQLDubiquitination[1]
338SPARKLLKLQLQNPPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. 
Sequence Annotation
 MOD_RES 28 28 Phosphoserine.
 MOD_RES 30 30 Phosphoserine.
 MOD_RES 58 58 Phosphoserine.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 81 81 Phosphoserine.  
Keyword
 Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Complete proteome; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 356 AA 
Protein Sequence
MTSVTRSEII DEKGPVMSKT HDHQLESSLS PVEVFAKTSA SLEMNQGVSE ERIHLGSSPK 60
KGGNCDLSHQ ERLQSKSLHL SPQEQSASYQ DRRQSWRRAS MKETNRRKSL HPIHQGITEL 120
SRSISVDLAE SKRLGCLLLS SFQFSIQKLE PFLRDTKGFS LESFRAKASS LSEELKHFAD 180
GLETDGTLQK CFEDSNGKAS DFSLEASVAE MKEYITKFSL ERQTWDQLLL HYQQEAKEIL 240
SRGSTEAKIT EVKVEPMTYL GSSQNEVLNT KPDYQKILQN QSKVFDCMEL VMDELQGSVK 300
QLQAFMDEST QCFQKVSVQL GKRSMQQLDP SPARKLLKLQ LQNPPAIHGS GSGSCQ 356 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000444; C:MIS12/MIND type complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 
Interpro
 IPR013218; Mtw1_DSN1. 
Pfam
 PF08202; Mis12_component 
SMART
  
PROSITE
  
PRINTS