CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003874
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein transport protein SEC7 
Protein Synonyms/Alias
  
Gene Name
 SEC7 
Gene Synonyms/Alias
 YDR170C; YD9395.01C; YD9489.05C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
64AGSTAETKETSNDATubiquitination[1]
75NDATNGMKTPEETEDubiquitination[1]
86ETEDTNDKRHDDEGEubiquitination[1]
513VMAKICAKPLETELDubiquitination[1]
764PNTHTANKVLLNTTSubiquitination[1]
797MTSNDDFKPTYEDEEubiquitination[1, 2]
1053EAYNQVSKEISSKTEubiquitination[1]
1237SFFDVWGKKATPTELacetylation[3]
1238FFDVWGKKATPTELAacetylation[3]
1238FFDVWGKKATPTELAubiquitination[1]
1487TKNKRFQKLSLHALEacetylation[4]
1780GNSVPTVKDELNPKPubiquitination[1]
1786VKDELNPKPASLSIPubiquitination[1]
1859RITRLLEKSYEFSRDubiquitination[1]
1885VEARVVDKIPNLLKQubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [4] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 May play a role in vesicular budding and traffic between compartments of the Golgi apparatus. 
Sequence Annotation
 DOMAIN 824 1010 SEC7.
 MOD_RES 212 212 Phosphoserine.
 MOD_RES 215 215 Phosphoserine.
 MOD_RES 334 334 Phosphothreonine.
 MOD_RES 447 447 Phosphoserine.
 MOD_RES 452 452 Phosphoserine.
 MOD_RES 455 455 Phosphoserine.
 MOD_RES 807 807 Phosphoserine.
 MOD_RES 1226 1226 Phosphoserine.
 MOD_RES 1240 1240 Phosphothreonine.
 MOD_RES 1741 1741 Phosphoserine.
 MOD_RES 1752 1752 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Golgi apparatus; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2009 AA 
Protein Sequence
MSEQNSVVNA EKGDGEISSN VETASSVNPS VKPQNAIKEE AKETNGEDQK CKGPENAGST 60
AETKETSNDA TNGMKTPEET EDTNDKRHDD EGEDGDEDED EDEDEDEDNG DEDDEDVDSS 120
SSETSSEDGE DSESVSGEST ESSSGEDEES DESDGNTSNS SSGDESGSEE EEEEEEEEEE 180
EENAGEPAIA HQDSVPTNDS TAPRSTHTRN ISLSSNGSNT NSTIILVKTT LETILNDKDI 240
KKNSNAQKAI ERTLQKFKEF DPQTTNNPHY VDSILVFEAL RASCRTKSSK VQSLALDCLS 300
KLFSFRSLDE TLLVNPPDSL ASNDQRQDAA DGITPPPKQK IIDAAIDTIS DCFQGEGTDD 360
RVELQIVRAL SSCILEEDSS SLCHGASLLK AIRTIYNVFV FSLNPSNQGI AQATLTQIIS 420
SVYDKIDLKQ STSSAVSLST KNHQQQSAIE LSEASENAET PAPLTLENMD KLNDDEERLM 480
DAQQPDSIAI TNQDLAVKDA FLVFRVMAKI CAKPLETELD MRSHAVRSKL LSLHIIYSII 540
KDHIDVFLSH NIFLPGKERV CFIDSIRQYL RLVLSRNAAS PLAPVFEVTL EIMWLLIANL 600
RADFVKEIPV FLTEIYFPIS ELTTSTSQQK RYFLSVIQRI CNDPRTLVEF YLNYDCNPGM 660
PNVMEITVDY LTRLALTRVE ITQTQRSYYD EQISKSLSTY NFSQLPLLTS SNLSSSPDVG 720
QVNLLFPLDF ALKMVSLNCI VSVLRSLSSW AHKALNPNTH TANKVLLNTT SSARQESRSS 780
LSNDVRSSIM TSNDDFKPTY EDEESRSLSS QNIDADDPTQ FENLKLRKTA LSECIAIFNN 840
KPKKAIPVLI KKGFLKDDSP ISIAKWLLET EGLDMAAVGD YLGEGDDKNI AIMHAFVDEF 900
DFTGMSIVDA LRSFLQSFRL PGEGQKIDRF MLKFAERFVD QNPGVFSKAD TAYVLSYSLI 960
MLNTDLHSSQ IKNKMSLQEF LENNEGIDNG RDLPRDFLEG LFNEIANNEI KLISEQHQAM 1020
LSGDTNLVQQ QQSAFNFFNS RDLTREAYNQ VSKEISSKTE LVFKNLNKNK GGPDVYYAAS 1080
HVEHVKSIFE TLWMSFLAAL TPPFKDYDDI DTTNKCLEGL KISIKIASTF RINDARTSFV 1140
GALVQFCNLQ NLEEIKVKNV NAMVILLEVA LSEGNYLEGS WKDILLVVSQ MERLQLISKG 1200
IDRDTVPDVA QARVANPRVS YESSRSNNTS FFDVWGKKAT PTELAQEKHH NQTLSPEISK 1260
FISSSELVVL MDNIFTKSSE LSGNAIVDFI KALTAVSLEE IESSENASTP RMFSLQKMVD 1320
VCYYNMDRIK LEWTPLWAVM GKAFNKIATN SNLAVVFFAI DSLRQLSMRF LDIEELSGFE 1380
FQHDFLKPFE YTVQNSGNTE VQEMIIECFR NFILTKSESI KSGWKPILES LQYTARSSTE 1440
SIVLKTQLLV SNDIVTNHFE NVFSQEDAFS ELVGVFREIT KNKRFQKLSL HALESLRKMT 1500
QNVADICFYN ENKTEEERKH NDALLRGKDI FQDVWFPMLF CFNDTIMTAE DLEVRSRALN 1560
YMFDALVAYG GKFNDDFWEK ICKKLLFPIF GVLSKHWEVN QFNSHDDLSV WLSTTLIQAL 1620
RNLIALFTHY FESLNRMLDG FLGLLVSCIC QENDTIARIG RSCLQQLILQ NVSKFNEYHW 1680
NQIGDVFDKL FDLTTANELF DYDPLQQGRK SSVSHHQTTN DTSQHSDDDS NDRRENDSNI 1740
SETVERAHQE ESSEDVGGDM VETLNGQTKL NNGNSVPTVK DELNPKPASL SIPKKTKHMK 1800
RNESNEDIRR RINIKNSIVV KCVLQLLMIE LLNELFENED FAHCIPYKEA IRITRLLEKS 1860
YEFSRDFNED YGLRTRLVEA RVVDKIPNLL KQETSAAAVL LDIMFQLYLN DDEKKADLIT 1920
RLITICIQVV EGYVSLDDRT MERSINAWRS VIVEILQGYY EFDDEDFRLY CPAMYALVIQ 1980
ILDKSVPTEL RHAIKQFLSR VGELYLSTD 2009 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005798; C:Golgi-associated vesicle; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005770; C:late endosome; IDA:SGD.
 GO:0005802; C:trans-Golgi network; IDA:SGD.
 GO:0005086; F:ARF guanyl-nucleotide exchange factor activity; IDA:SGD.
 GO:0000045; P:autophagic vacuole assembly; IMP:SGD.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD.
 GO:0006891; P:intra-Golgi vesicle-mediated transport; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR015403; DUF1981_SEC7_assoc.
 IPR000904; Sec7.
 IPR023394; SEC7_alpha_orthog. 
Pfam
 PF09324; DUF1981
 PF01369; Sec7 
SMART
 SM00222; Sec7 
PROSITE
 PS50190; SEC7 
PRINTS