CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005616
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar membrane protein PEP3 
Protein Synonyms/Alias
 Carboxypeptidase Y-deficient protein 3; Vacuolar morphogenesis protein 8; Vacuolar protein sorting-associated protein 18; Vacuolar protein-targeting protein 18 
Gene Name
 PEP3 
Gene Synonyms/Alias
 VAM8; VPS18; VPT18; YLR148W; L9634.2 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
915IDETELAKWNE****ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. 
Sequence Annotation
 REPEAT 585 741 CHCR.
 ZN_FING 826 851 RING-type; atypical.
 MOD_RES 907 907 Phosphoserine.  
Keyword
 Complete proteome; Membrane; Metal-binding; Phosphoprotein; Protein transport; Reference proteome; Transport; Vacuole; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 918 AA 
Protein Sequence
MIKTRIEEVQ LQFLTGNTEL THLKVSNDQL IVTTQRTIYR INLQDPAIVN HFDCPLSKEL 60
ETIMNVHVSP MGSVILIRTN FGRYMLLKDG EFTQLNKIKN LDLSSLHWIN ETTFLMGIKK 120
TPKLYRVELT GKDITTKLWY ENKKLSGGID GIAYWEGSLL LTIKDNILYW RDVTNMKFPL 180
VLPDESEQFE RLKHHAIKKF DSYNGLFAWV TSNGIVFGDL KEKQMEKDPA SNNFGKFLSS 240
SKVLLNFELP DYQNDKDHLI KDIVLTAFHI LLLRKNTVTM VSQLNNDVVF HETIPRHQLT 300
GSNTDSNEKF LGLVRDSVKE TFWCFSNINV FEIIIENEPN SVWNLLVRDN KFDKALSLKG 360
LTVREIESVK LSKAMYLFHT AKDFHSAAQT LGSMKDLSHF GEIALNFLQI KDYNDLNVIL 420
IKQLDNVPWK STQVVLSSWI IWNFMKQLND IELKINTTKP ASTDEDNLLN WNLNLKEKSN 480
ELTKFLESHL EKLDNETVYQ IMSKQNRQNE LLIFASLIND MKFLLSFWID QGNWYESLKI 540
LLTINNHDLV YKYSLILLLN SPEATVSTWM KIKDLDPNKL IPTILKFFTN WQNNSKLITN 600
ISEYPENYSL TYLKWCVREV PKMCNPIVYN SILYMMITDP RNDMILENDI IKFMKSNENK 660
YDLNFQLRLS LKFKKTKTSI FLLTRLNLFE DAIDLALKNN LIDDCKVIVN DEILIEDYKL 720
RKRLWLKIAK HLLLSMKDID IKQLIRTILN DSNEILTIKD LLPFFNEYTT IANLKEELIK 780
FLENHNMKMN EISEDIINSK NLKVEINTEI SKFNEIYRIL EPGKSCDECG KFLQIKKFIV 840
FPCGHCFHWN CIIRVILNSN DYNLRQKTEN FLKAKSKHNL NDLENIIVEK CGLCSDININ 900
KIDQPISIDE TELAKWNE 918 
Gene Ontology
 GO:0033263; C:CORVET complex; IDA:SGD.
 GO:0000306; C:extrinsic to vacuolar membrane; IDA:SGD.
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0030897; C:HOPS complex; IPI:SGD.
 GO:0034515; C:proteasome storage granule; IC:SGD.
 GO:0030674; F:protein binding, bridging; IMP:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006895; P:Golgi to endosome transport; IGI:SGD.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0045324; P:late endosome to vacuole transport; IGI:SGD.
 GO:0035542; P:regulation of SNARE complex assembly; IDA:SGD.
 GO:0032889; P:regulation of vacuole fusion, non-autophagic; IDA:SGD.
 GO:0042144; P:vacuole fusion, non-autophagic; IDA:SGD.
 GO:0006904; P:vesicle docking involved in exocytosis; IMP:SGD. 
Interpro
 IPR000547; Clathrin_H-chain/VPS_repeat.
 IPR007810; Pep3_Vps18.
 IPR019453; VPS39/TGF_beta_rcpt-assoc_2.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00637; Clathrin
 PF05131; Pep3_Vps18
 PF10367; Vps39_2 
SMART
 SM00299; CLH
 SM00184; RING 
PROSITE
 PS50236; CHCR
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS