CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031020
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear pore complex protein Nup85 
Protein Synonyms/Alias
 cDNA FLJ53138, highly similar to Homo sapiens pericentrin 1 (PCNT1), mRNA 
Gene Name
 NUP85 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13PFIYIIRKDVDVYSQubiquitination[1, 2, 3]
24VYSQILRKLFNESHGubiquitination[1, 2, 3, 4, 5]
46IDEELTGKSRKSQLVubiquitination[2, 3, 4, 5, 6, 7]
78HQVAIAAKDPANGRQubiquitination[3, 4, 7]
172EARQMLSKEADASPAubiquitination[3, 4, 7]
211TLTELELKWQHWHEEubiquitination[3]
403IPLNTEQKALKVLRIubiquitination[2, 3, 4, 5, 6, 7]
406NTEQKALKVLRICEQubiquitination[3, 4, 7]
425EQVRSICKILAMKAVubiquitination[3, 4]
430ICKILAMKAVRNNRLubiquitination[3, 4, 5]
448LSWSIRAKDAAFATLubiquitination[2, 3, 4, 5]
494DRLTFLGKYREFHRMubiquitination[1, 3, 4, 5]
585DDDIETTKVEMLRLSubiquitination[2, 3, 4, 5, 8]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 610 AA 
Protein Sequence
MVPSCPFIYI IRKDVDVYSQ ILRKLFNESH GIFLGLQRID EELTGKSRKS QLVRVSKNYR 60
SVIRACMEEM HQVAIAAKDP ANGRQFSSQV SILSAMELIW NLCEILFIEV APAGPLLLHL 120
LDWVRLHVCE VDSLSADVLG SENPSKHDSF WNLVTILVLQ GRLDEARQML SKEADASPAS 180
AGICRIMGDL MRTMPILSPG NTQTLTELEL KWQHWHEECE RYLQDSTFAT SPHLESLLKI 240
MLGDEAALLE QKELLSNWYH FLVTRLLYSN PTVKPIDLHY YAQSSLDLFL GGESSPEPLD 300
NILLAAFEFD IHQVIKECSI ALSNWWFVAH LTDLLDHCKL LQSHNLYFGS NMREFLLLEY 360
ASGLFAHPSL WQLGVDYFDY CPELGRVSLE LHIERIPLNT EQKALKVLRI CEQRQMTEQV 420
RSICKILAMK AVRNNRLGSA LSWSIRAKDA AFATLVSDRF LRDYCERGCF SDLDLIDNLG 480
PAMMLSDRLT FLGKYREFHR MYGEKRFADA ASLLLSLMTS RIAPRSFWMT LLTDALPLLE 540
QKQVIFSAEQ TYELMRCLED LTSRRPVHGE SDTEQLQDDD IETTKVEMLR LSLARNLARA 600
IIREGSLEGS 610 
Gene Ontology
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0048246; P:macrophage chemotaxis; IEA:Compara. 
Interpro
 IPR011502; Nucleoporin_Nup85. 
Pfam
 PF07575; Nucleopor_Nup85 
SMART
  
PROSITE
  
PRINTS