CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003168
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycine cleavage system transcriptional activator 
Protein Synonyms/Alias
 Gcv operon activator 
Gene Name
 gcvA 
Gene Synonyms/Alias
 b2808; JW2779 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
51LEDFLGLKLFRRRNRacetylation[1]
188KTPEDLAKHTLLHDAacetylation[1]
291FRQWILAKAAAEQEKacetylation[1]
298KAAAEQEKFRFRYEQacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Binds to three sites upstream of the gcv promoter. 
Sequence Annotation
 DOMAIN 6 63 HTH lysR-type.
 DNA_BIND 23 42 H-T-H motif (By similarity).  
Keyword
 Activator; Complete proteome; Cytoplasm; DNA-binding; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 305 AA 
Protein Sequence
MSKRLPPLNA LRVFDAAARH LSFTRAAEEL FVTQAAVSHQ IKSLEDFLGL KLFRRRNRSL 60
LLTEEGQSYF LDIKEIFSQL TEATRKLQAR SAKGALTVSL LPSFAIHWLV PRLSSFNSAY 120
PGIDVRIQAV DRQEDKLADD VDVAIFYGRG NWPGLRVEKL YAEYLLPVCS PLLLTGEKPL 180
KTPEDLAKHT LLHDASRRDW QTYTRQLGLN HINVQQGPIF SHSAMVLQAA IHGQGVALAN 240
NVMAQSEIEA GRLVCPFNDV LVSKNAFYLV CHDSQAELGK IAAFRQWILA KAAAEQEKFR 300
FRYEQ 305 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IDA:EcoliWiki.
 GO:0006351; P:transcription, DNA-dependent; IDA:EcoCyc. 
Interpro
 IPR005119; LysR_subst-bd.
 IPR000847; Tscrpt_reg_HTH_LysR.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00126; HTH_1
 PF03466; LysR_substrate 
SMART
  
PROSITE
 PS50931; HTH_LYSR 
PRINTS
 PR00039; HTHLYSR.