UniProt Accession

 YAFO_ECOLI; Q47157 

Genbank Protein ID

 D38582; U70214; U00096; AP009048 

Genbank Nucleotide ID

 BAA07595.1; AAB08653.1; AAC73337.1; BAA77902.1 

Protein Name

 mRNA interferase YafO 

Protein Synonyms/Alias

 Endoribonuclease YafO; Toxin YafO 

Gene Name

 yafO 

Gene Synonyms/Alias

 b0233; JW0223 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
123	NQMHKIGKMAEAFRM	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Toxic component of a toxin-antitoxin (TA) module. A translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin YafN. Overexpression causes cleavage of a number of mRNAs in a ribosome-dependent fashion. YafO binding to the 50S ribosomal subunit in the translation complex induces mRNA cleavage 3' to the region protected by the ribosome; YafO alone is not able to digest mRNA. 

Sequence Annotation

  

Keyword

 Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease; Reference proteome; RNA-binding; SOS response; Stress response; Toxin. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 132 AA 

Protein Sequence

Gene Ontology

 GO:0004521; F:endoribonuclease activity; IDA:UniProtKB.
 GO:0043023; F:ribosomal large subunit binding; IDA:EcoCyc.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0017148; P:negative regulation of translation; IDA:EcoCyc.
 GO:0009432; P:SOS response; IEA:UniProtKB-KW. 

Interpro

 IPR020353; Toxin_YafO. 

Pfam

 PF13957; YafO_toxin 

SMART

  

PROSITE

  

PRINTS