CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012902
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable pre-mRNA-splicing factor ATP-dependent RNA helicase 
Protein Synonyms/Alias
  
Gene Name
 At3g26560; MFE16.8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
505PTFGQRSKLSIQEQRsumoylation[1]
Reference
 [1] Proteomic analyses identify a diverse array of nuclear processes affected by small ubiquitin-like modifier conjugation in Arabidopsis.
 Miller MJ, Barrett-Wilt GA, Hua Z, Vierstra RD.
 Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16512-7. [PMID: 20813957
Functional Description
 Could act late in the splicing of pre-mRNA and mediate the release of the spliced mRNA from spliceosomes (By similarity). 
Sequence Annotation
 DOMAIN 214 283 S1 motif.
 DOMAIN 525 688 Helicase ATP-binding.
 DOMAIN 706 886 Helicase C-terminal.
 NP_BIND 538 545 ATP (By similarity).
 MOTIF 635 638 DEAH box.
 MOD_RES 411 411 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1168 AA 
Protein Sequence
MEKEELNKLN HLSLVSNVCN ELETHLGSAE KVLAEFIIDL GRHSETVDEF DKNLKEAGAE 60
MPDYFVRSLL TTIHGIYPPK PKSEKKKEEG DDQKFKGLAI KDTKDKVKEL EKEIEREAEE 120
RRREEDRNRD RDRRESGRDR DRDRNRDRDD RRDRHRDRER NRGDEEGEDR RSDRRHRERG 180
RGDGGEGEDR RRDRRAKDEY VEEDKGGANE PELYQVYKGR VTRVMDAGCF VQFDKFRGKE 240
GLVHVSQMAT RRVDKAKEFV KRDMEVYVKV ISISSDKYSL SMRDVDQNTG RDLIPLRKPS 300
DEDDSSRSNP SYRTKDGQVT KTGISGIRIV EENDVAPSRR PLKKMSSPER WEAKQLIASG 360
VLRVDEFPMY DEDGDGMLYQ EEGAEEELEI EMNEDEPAFL QGQTRYSVDM SPVKIFKNPE 420
GSLSRAAALQ SALTKERREM REQQQRTMLD SIPKDLNRPW EDPMPETGER HLAQELRGVG 480
LSAYDMPEWK KDAFGKTPTF GQRSKLSIQE QRESLPIYKL KKELIQAVHD NQVLVVIGET 540
GSGKTTQVTQ YLAEAGYTTK GKIGCTQPRR VAAMSVAKRV AEEFGCRLGE EVGYAIRFED 600
CTGPDTVIKY MTDGMLLREI LIDENLSQYS VIMLDEAHER TIHTDVLFGL LKKLMKRRLD 660
LRLIVTSATL DAEKFSGYFF NCNIFTIPGR TFPVEILYTK QPETDYLDAA LITVLQIHLT 720
EPEGDILVFL TGQEEIDSAC QSLYERMKGL GKNVPELIIL PVYSALPSEM QSRIFDPPPP 780
GKRKVVVATN IAEASLTIDG IYYVVDPGFA KQNVYNPKQG LESLVITPIS QASAKQRAGR 840
AGRTGPGKCY RLYTESAYRN EMPPTSIPEI QRINLGMTTL TMKAMGINDL LSFDFMDPPQ 900
PQALISAMEQ LYSLGALDEE GLLTKLGRKM AEFPLEPPLS KMLLASVDLG CSDEILTMIA 960
MIQTGNIFYR PREKQAQADQ KRAKFFQPEG DHLTLLAVYE AWKAKNFSGP WCFENFIQSR 1020
SLRRAQDVRK QLLSIMDKYK LDVVTAGKNF TKIRKAITAG FFFHGARKDP QEGYRTLVEN 1080
QPVYIHPSSA LFQRQPDWVI YHDLVMTTKE YMREVTVIDP KWLVELAPRF FKVSDPTKMS 1140
KRKRQERIEP LYDRYHEPNS WRLSKRRA 1168 
Gene Ontology
 GO:0005829; C:cytosol; IDA:TAIR.
 GO:0005739; C:mitochondrion; IDA:TAIR.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0009506; C:plasmodesma; IDA:TAIR.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase.
 IPR003029; Rbsml_prot_S1_RNA-bd_dom.
 IPR022967; RNA-binding_domain_S1. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind
 PF00575; S1 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc
 SM00316; S1 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS50126; S1 
PRINTS