CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019595
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium/calmodulin-dependent protein kinase kinase 2 
Protein Synonyms/Alias
 CaM-KK 2; CaM-kinase kinase 2; CaMKK 2; Calcium/calmodulin-dependent protein kinase kinase beta; CaM-KK beta; CaM-kinase kinase beta; CaMKK beta 
Gene Name
 CAMKK2 
Gene Synonyms/Alias
 CAMKKB; KIAA0787 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
173TLKDEIGKGSYGVVKubiquitination[1]
241YQEIAILKKLDHPNVubiquitination[1]
314KIIHRDIKPSNLLVGubiquitination[1]
338FGVSNEFKGSDALLSubiquitination[1]
403MCLHSKIKSQALEFPubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin- binding domain are inactive. Efficiently phosphorylates 5'-AMP- activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching. 
Sequence Annotation
 DOMAIN 165 446 Protein kinase.
 NP_BIND 171 179 ATP (By similarity).
 REGION 204 226 RP domain.
 REGION 472 477 Autoinhibitory domain (By similarity).
 REGION 475 500 Calmodulin-binding (By similarity).
 ACT_SITE 312 312 Proton acceptor (By similarity).
 BINDING 194 194 ATP (By similarity).
 MOD_RES 129 129 Phosphoserine (By similarity).
 MOD_RES 133 133 Phosphoserine (By similarity).
 MOD_RES 495 495 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Calmodulin-binding; Cell projection; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 588 AA 
Protein Sequence
MSSCVSSQPS SNRAAPQDEL GGRGSSSSES QKPCEALRGL SSLSIHLGME SFIVVTECEP 60
GCAVDLGLAR DRPLEADGQE VPLDTSGSQA RPHLSGRKLS LQERSQGGLA AGGSLDMNGR 120
CICPSLPYSP VSSPQSSPRL PRRPTVESHH VSITGMQDCV QLNQYTLKDE IGKGSYGVVK 180
LAYNENDNTY YAMKVLSKKK LIRQAGFPRR PPPRGTRPAP GGCIQPRGPI EQVYQEIAIL 240
KKLDHPNVVK LVEVLDDPNE DHLYMVFELV NQGPVMEVPT LKPLSEDQAR FYFQDLIKGI 300
EYLHYQKIIH RDIKPSNLLV GEDGHIKIAD FGVSNEFKGS DALLSNTVGT PAFMAPESLS 360
ETRKIFSGKA LDVWAMGVTL YCFVFGQCPF MDERIMCLHS KIKSQALEFP DQPDIAEDLK 420
DLITRMLDKN PESRIVVPEI KLHPWVTRHG AEPLPSEDEN CTLVEVTEEE VENSVKHIPS 480
LATVILVKTM IRKRSFGNPF EGSRREERSL SAPGNLLTKK PTRECESLSE LKEARQRRQP 540
PGHRPAPRGG GGSALVRGSP CVESCWAPAP GSPARMHPLR PEEAMEPE 588 
Gene Ontology
 GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0005516; F:calmodulin binding; TAS:UniProtKB.
 GO:0004683; F:calmodulin-dependent protein kinase activity; IEA:EC.
 GO:0004713; F:protein tyrosine kinase activity; TAS:UniProtKB.
 GO:0019722; P:calcium-mediated signaling; TAS:UniProtKB.
 GO:0000165; P:MAPK cascade; TAS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; TAS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:0045859; P:regulation of protein kinase activity; TAS:UniProtKB. 
Interpro
 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS