CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032291
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GEM-interacting protein 
Protein Synonyms/Alias
 GMIP protein 
Gene Name
 GMIP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
91DLRLIRTKGGVDAALubiquitination[1, 2]
117ELLAWTEKRASYELEubiquitination[1]
184YYQPLAAKRTEIEKWubiquitination[1, 3]
196EKWRKEFKEQWMKEQubiquitination[1]
248QASPGPSKQQERRRRubiquitination[1]
289QQDLEIAKQRIVSHVubiquitination[1]
598HDVSSVLKRFLQELTubiquitination[4]
646PEVIRSLKTLLVQLPubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 944 AA 
Protein Sequence
MDAAEPGLPP GPEGRKRYSD IFRSLDNLEI SLGNVTLEML AGDPLLSEDP EPDKTPTATV 60
TNEASCWSGP SPEGPVPLTG EELDLRLIRT KGGVDAALEY AKTWSRYAKE LLAWTEKRAS 120
YELEFAKSTM KIAEAGKVSI QQQSHMPLQY IYTLFLEHDL SLGTLAMETV AQQKRDYYQP 180
LAAKRTEIEK WRKEFKEQWM KEQKRMNEAV QALRRAQLQY VQRSEDLRAR SQGSPEDSAP 240
QASPGPSKQQ ERRRRSREEA QAKAQEAEAL YQACVREANA RQQDLEIAKQ RIVSHVRKLV 300
FQGDEVLRRV TLSLFGLRGA QAERGPRAFA ALAECCAPFE PGQRYQEFVR ALRPEAPPPP 360
PPAFSFQEFL PSLNSSPLDI RKKLSGPLPP RLDENSAEPG PWEDPGTGWR WQGTPGPTPG 420
SDVDSVGGGS ESRSLDSPTS SPDLGDGLEN GLGSPFGKWT LSSAAQTHQL RRLRGPAKCR 480
ECEAFMVSGT ECEECFLTCH KRCLETLLIL CGHRRLPART PLFGVDFLQL PRDFPEEVPF 540
VVTKCTAEIE HRALDVQGIY RVSGSRVRVE RLCQAFENGR ALVELSGNSP HDVSSVLKRF 600
LQELTEPVIP FHLYDAFISL AKTLHADPGD DPGTPSPSPE VIRSLKTLLV QLPDSNYNTL 660
RHLVAHLFRV AARFMENKMS ANNLGIVFGP TLLRPPDGPR AASAIPVTCL LDSGHQAQLV 720
EFLIVHYEQI FGMDELPQAT EPPPQDSSPA PGPLTTSSQP PPPHLDPDSQ PPVLASDPGP 780
DPQHHSTLEQ HPTATPTEIP TPQSDQREDV AEDTKDGGGE VSSQGPEDSL LGTQSRGHFS 840
RQPVKYPRGG VRPVTHQLSS LALVASKLCE ETPITSVPRG SLRGRGPSPA AASPEGSPLR 900
RTPLPKHFEI TQETARLLSK LDSEAVPRAT CCPDVQPEEA EDHL 944 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro. 
Interpro
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00620; RhoGAP 
SMART
 SM00109; C1
 SM00324; RhoGAP 
PROSITE
 PS50238; RHOGAP
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS