UniProt Accession

 MOT1_HUMAN; P53985; Q5T8R6; Q9NSJ9 

Genbank Protein ID

 L31801; AJ438945; AL162079; AL158844; CH471122; BC026317 

Genbank Nucleotide ID

 AAC41707.1; CAD27707.1; CAB82412.1; CAI21872.1; EAW56552.1; AAH26317.1 

Protein Name

 Monocarboxylate transporter 1 

Protein Synonyms/Alias

 MCT 1; Solute carrier family 16 member 1 

Gene Name

 SLC16A1 

Gene Synonyms/Alias

 MCT1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
216	KSKASLEKAGKSGVK	ubiquitination	[1, 2]
223	KAGKSGVKKDLHDAN	ubiquitination	[2]
224	AGKSGVKKDLHDANT	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9, 10]
239	DLIGRHPKQEKRSVF	ubiquitination	[2, 5, 9, 10]
450	INYRLLAKEQKANEQ	ubiquitination	[1]
459	QKANEQKKESKEEET	ubiquitination	[1, 2, 5]
462	NEQKKESKEEETSID	acetylation	[11]
462	NEQKKESKEEETSID	ubiquitination	[1, 2, 5, 6]
473	TSIDVAGKPNEVTKA	ubiquitination	[1, 2, 5, 6, 7, 9, 10]
479	GKPNEVTKAAESPDQ	ubiquitination	[1, 2, 4, 5]
487	AAESPDQKDTDGGPK	ubiquitination	[1, 2, 9, 10, 12]
494	KDTDGGPKEEESPV*	methylation	[13]
494	KDTDGGPKEEESPV*	ubiquitination	[1, 2, 10]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [10] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [11] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [12] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [13] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 

Functional Description

 Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. 

Sequence Annotation

 MOD_RES 210 210 Phosphoserine (By similarity).
 MOD_RES 213 213 Phosphoserine.
 MOD_RES 461 461 Phosphoserine.
 MOD_RES 466 466 Phosphothreonine.
 MOD_RES 467 467 Phosphoserine.
 MOD_RES 483 483 Phosphoserine.
 MOD_RES 498 498 Phosphoserine.  

Keyword

 Cell membrane; Complete proteome; Disease mutation; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 500 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0015130; F:mevalonate transmembrane transporter activity; TAS:ProtInc.
 GO:0015355; F:secondary active monocarboxylate transmembrane transporter activity; IEA:InterPro.
 GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0006090; P:pyruvate metabolic process; TAS:Reactome. 

Interpro

 IPR011701; MFS.
 IPR020846; MFS_dom.
 IPR016196; MFS_dom_general_subst_transpt.
 IPR004743; Monocarb_transpt. 

Pfam

 PF07690; MFS_1 

SMART

  

PROSITE

 PS50850; MFS 

PRINTS