UniProt Accession

 IDHC_RAT; P41562; P80300 

Genbank Protein ID

 L35317 

Genbank Nucleotide ID

 AAA59356.1 

Protein Name

 Isocitrate dehydrogenase [NADP] cytoplasmic 

Protein Synonyms/Alias

 IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase 

Gene Name

 Idh1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
58	ATNDQVTKDAAEAIK	acetylation	[1]
65	KDAAEAIKKYNVGVK	acetylation	[1]
66	DAAEAIKKYNVGVKC	acetylation	[1]
81	ATITPDEKRVEEFKL	acetylation	[1]
87	EKRVEEFKLKQMWKS	acetylation	[1]
93	FKLKQMWKSPNGTIR	acetylation	[1]
126	RLVTGWVKPIIIGRH	acetylation	[1]
151	FVVPGPGKVEITYTP	acetylation	[1]
159	VEITYTPKDGSQKVT	acetylation	[1]
217	STKNTILKKYDGRFK	acetylation	[1]
224	KKYDGRFKDIFQEIY	acetylation	[1]
233	IFQEIYDKQYKSKFE	acetylation	[1]
238	YDKQYKSKFEAQKIW	acetylation	[1]
243	KSKFEAQKIWYEHRL	acetylation	[1]
321	RHYRMYQKGQETSTN	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

  

Sequence Annotation

 NP_BIND 75 77 NADP (By similarity).
 NP_BIND 310 315 NADP (By similarity).
 REGION 94 100 Substrate binding (By similarity).
 METAL 252 252 Magnesium or manganese (By similarity).
 METAL 275 275 Magnesium or manganese (By similarity).
 BINDING 77 77 Substrate (By similarity).
 BINDING 82 82 NADP (By similarity).
 BINDING 109 109 Substrate (By similarity).
 BINDING 132 132 Substrate (By similarity).
 BINDING 260 260 NADP (By similarity).
 BINDING 328 328 NADP; via amide nitrogen and carbonyl
 MOD_RES 321 321 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Reference proteome; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 414 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005777; C:peroxisome; IDA:HGNC.
 GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IDA:RGD.
 GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB.
 GO:0008585; P:female gonad development; IEP:RGD.
 GO:0006749; P:glutathione metabolic process; IEA:Compara.
 GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
 GO:0006102; P:isocitrate metabolic process; ISS:UniProtKB.
 GO:0006979; P:response to oxidative stress; IEA:Compara.
 GO:0048545; P:response to steroid hormone stimulus; IDA:RGD.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 

Interpro

 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR004790; Isocitrate_DH_NADP.
 IPR024084; IsoPropMal-DH-like_dom. 

Pfam

 PF00180; Iso_dh 

SMART

  

PROSITE

 PS00470; IDH_IMDH 

PRINTS