CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021965
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA methyltransferase 1-associated protein 1 
Protein Synonyms/Alias
 DNMAP1; DNMT1-associated protein 1 
Gene Name
 DMAP1 
Gene Synonyms/Alias
 KIAA1425 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
35KDIINPDKKKSKKSSacetylation[1]
65YALLYSDKKDAPPLLubiquitination[2]
66ALLYSDKKDAPPLLPubiquitination[2]
106PFTNPARKDGAMFFHubiquitination[2]
192KRSVEDLKERYYHICubiquitination[2]
214AVPGTDLKIPVFDAGubiquitination[2]
301PQKKEAEKPAVPETAacetylation[3, 4, 5]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX- mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity. 
Sequence Annotation
 DOMAIN 149 199 SANT.
 MOD_RES 445 445 Phosphothreonine.
 MOD_RES 448 448 Phosphoserine.  
Keyword
 3D-structure; Activator; Chromatin regulator; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Growth regulation; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 467 AA 
Protein Sequence
MATGADVRDI LELGGPEGDA ASGTISKKDI INPDKKKSKK SSETLTFKRP EGMHREVYAL 60
LYSDKKDAPP LLPSDTGQGY RTVKAKLGSK KVRPWKWMPF TNPARKDGAM FFHWRRAAEE 120
GKDYPFARFN KTVQVPVYSE QEYQLYLHDD AWTKAETDHL FDLSRRFDLR FVVIHDRYDH 180
QQFKKRSVED LKERYYHICA KLANVRAVPG TDLKIPVFDA GHERRRKEQL ERLYNRTPEQ 240
VAEEEYLLQE LRKIEARKKE REKRSQDLQK LITAADTTAE QRRTERKAPK KKLPQKKEAE 300
KPAVPETAGI KFPDFKSAGV TLRSQRMKLP SSVGQKKIKA LEQMLLELGV ELSPTPTEEL 360
VHMFNELRSD LVLLYELKQA CANCEYELQM LRHRHEALAR AGVLGGPATP ASGPGPASAE 420
PAVTEPGLGP DPKDTIIDVV GAPLTPNSRK RRESASSSSS VKKAKKP 467 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0035267; C:NuA4 histone acetyltransferase complex; IDA:UniProtKB.
 GO:0005657; C:replication fork; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0001103; F:RNA polymerase II repressing transcription factor binding; IDA:UniProtKB.
 GO:0003714; F:transcription corepressor activity; IEA:Compara.
 GO:0006338; P:chromatin remodeling; IEA:InterPro.
 GO:0006306; P:DNA methylation; TAS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:InterPro.
 GO:0043968; P:histone H2A acetylation; IDA:UniProtKB.
 GO:0043967; P:histone H4 acetylation; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0042993; P:positive regulation of transcription factor import into nucleus; IDA:UniProtKB.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR008468; DMAP1.
 IPR001005; SANT/Myb.
 IPR027109; Swc4/Dmap1. 
Pfam
 PF05499; DMAP1 
SMART
 SM00717; SANT 
PROSITE
 PS51293; SANT 
PRINTS