CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003119
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chromosome initiation inhibitor 
Protein Synonyms/Alias
 OriC replication inhibitor 
Gene Name
 iciA 
Gene Synonyms/Alias
 b2916; JW2883 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
27GFERAAQKLCITQSAacetylation[1]
65RPTEQGQKLLALLRQacetylation[1]
170DYLFVSSKPFAEKYFacetylation[1]
175SSKPFAEKYFPNGVTacetylation[1]
281PESRMMRKVTDALLDacetylation[1]
292ALLDYGHKVLRQD**acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication. 
Sequence Annotation
 DOMAIN 4 60 HTH lysR-type.
 DNA_BIND 21 40 H-T-H motif (By similarity).  
Keyword
 Complete proteome; Direct protein sequencing; DNA-binding; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 297 AA 
Protein Sequence
MKRPDYRTLQ ALDAVIRERG FERAAQKLCI TQSAVSQRIK QLENMFGQPL LVRTVPPRPT 60
EQGQKLLALL RQVELLEEEW LGDEQTGSTP LLLSLAVNAD SLATWLLPAL APVLADSPIR 120
LNLQVEDETR TQERLRRGEV VGAVSIQHQA LPSCLVDKLG ALDYLFVSSK PFAEKYFPNG 180
VTRSALLKAP VVAFDHLDDM HQAFLQQNFD LPPGSVPCHI VNSSEAFVQL ARQGTTCCMI 240
PHLQIEKELA SGELIDLTPG LFQRRMLYWH RFAPESRMMR KVTDALLDYG HKVLRQD 297 
Gene Ontology
 GO:0003681; F:bent DNA binding; IDA:EcoliWiki.
 GO:0043565; F:sequence-specific DNA binding; IDA:EcoliWiki.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:EcoCyc.
 GO:0032297; P:negative regulation of DNA-dependent DNA replication initiation; IDA:EcoCyc.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:EcoCyc.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:EcoCyc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR023490; Chromosome_initiation-inh.
 IPR005119; LysR_subst-bd.
 IPR017685; Tscrpt_reg_ArgP.
 IPR000847; Tscrpt_reg_HTH_LysR.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00126; HTH_1
 PF03466; LysR_substrate 
SMART
  
PROSITE
 PS50931; HTH_LYSR 
PRINTS
 PR00039; HTHLYSR.