UniProt Accession

 NFE2_HUMAN; Q16621; Q07720; Q6ICV9 

Genbank Protein ID

 L24122; L13974; S77763; CR450284; BT007288; DQ367844; BC005044 

Genbank Nucleotide ID

 AAA16118.1; AAA35612.1; AAB34115.1; CAG29280.1; AAP35952.1; ABC79302.1; AAH05044.1 

Protein Name

 Transcription factor NF-E2 45 kDa subunit 

Protein Synonyms/Alias

 Leucine zipper protein NF-E2; Nuclear factor, erythroid-derived 2 45 kDa subunit; p45 NF-E2 

Gene Name

 NFE2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
368	FLVPRGTKMEATD**	sumoylation	[1]

Reference

 [1] Sumoylation of p45/NF-E2: nuclear positioning and transcriptional activation of the mammalian beta-like globin gene locus.
 Shyu YC, Lee TL, Ting CY, Wen SC, Hsieh LJ, Li YC, Hwang JL, Lin CC, Shen CK.
 Mol Cell Biol. 2005 Dec;25(23):10365-78. [PMID: 16287851] 

Functional Description

 Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. 

Sequence Annotation

 DOMAIN 266 329 bZIP.
 REGION 1 206 Transactivation domain.
 REGION 1 83 Required for interaction with MAPK8 (By
 REGION 268 287 Basic motif (By similarity).
 REGION 291 298 Leucine-zipper (By similarity).
 MOTIF 61 65 PXY motif 1.
 MOTIF 79 83 PXY motif 2.
 MOD_RES 157 157 Phosphoserine; by MAPK8 (By similarity).
 MOD_RES 170 170 Phosphoserine; by PKA (By similarity).
 CROSSLNK 368 368 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Activator; Complete proteome; Cytoplasm; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 373 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0043565; F:sequence-specific DNA binding; TAS:BHF-UCL.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0050699; F:WW domain binding; IDA:MGI.
 GO:0008015; P:blood circulation; TAS:ProtInc.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007267; P:cell-cell signaling; IEA:Compara.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0030502; P:negative regulation of bone mineralization; IEA:Compara.
 GO:0006337; P:nucleosome disassembly; TAS:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; TAS:BHF-UCL.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; TAS:BHF-UCL. 

Interpro

 IPR004827; bZIP.
 IPR008917; Euk_TF_DNA-bd. 

Pfam

 PF00170; bZIP_1 

SMART

 SM00338; BRLZ 

PROSITE

 PS50217; BZIP
 PS00036; BZIP_BASIC 

PRINTS