CPLM-019525

Genbank Nucleotide ID

RNA-binding protein 14

Protein Synonyms/Alias

Paraspeckle protein 2; PSP2; RNA-binding motif protein 14; RRM-containing coactivator activator/modulator; Synaptotagmin-interacting protein; SYT-interacting protein

Homo sapiens (Human)

Position	Peptide	Type	References
148	GKEGHWSKECPIDRS	ubiquitination	[1, 2, 3]
198	GALDAYYKRCRAARS	ubiquitination	[1, 4]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]

Functional Description

Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.

DOMAIN 1 73 RRM 1.
DOMAIN 79 149 RRM 2.
REGION 307 354 TRBP-interacting domain.
MOD_RES 161 161 Phosphoserine.
MOD_RES 206 206 Phosphothreonine.
MOD_RES 220 220 Phosphoserine.
MOD_RES 256 256 Phosphoserine.
MOD_RES 520 520 Phosphoserine (By similarity).
MOD_RES 562 562 Phosphoserine.
MOD_RES 572 572 Phosphothreonine.
MOD_RES 582 582 Phosphoserine (By similarity).
MOD_RES 618 618 Phosphoserine.
MOD_RES 629 629 Phosphothreonine (By similarity).
MOD_RES 632 632 Phosphoserine (By similarity).

3D-structure; Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; RNA-binding; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016592; C:mediator complex; NAS:UniProtKB.
GO:0030529; C:ribonucleoprotein complex; TAS:UniProtKB.
GO:0005667; C:transcription factor complex; IPI:UniProtKB.
GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; IPI:UniProtKB.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0030674; F:protein binding, bridging; NAS:UniProtKB.
GO:0003723; F:RNA binding; NAS:UniProtKB.
GO:0001104; F:RNA polymerase II transcription cofactor activity; NAS:UniProtKB.
GO:0006310; P:DNA recombination; NAS:UniProtKB.
GO:0006281; P:DNA repair; NAS:UniProtKB.
GO:0006260; P:DNA replication; NAS:UniProtKB.
GO:0042921; P:glucocorticoid receptor signaling pathway; NAS:UniProtKB.
GO:0016575; P:histone deacetylation; IPI:UniProtKB.
GO:0030520; P:intracellular estrogen receptor signaling pathway; NAS:UniProtKB.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
GO:0009725; P:response to hormone stimulus; TAS:UniProtKB.

IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.