UniProt Accession

 DHRS2_HUMAN; Q13268; D3DS54; Q53GS4; Q7Z789; Q9H2R2 

Genbank Protein ID

 U31875; AF244132; AK222857; AL135999; CH471078; CH471078; BC002786; BC007339 

Genbank Nucleotide ID

 AAA82048.1; AAG33703.1; BAD96577.1; EAW66130.1; EAW66131.1; AAH02786.1; AAH07339.2 

Protein Name

 Dehydrogenase/reductase SDR family member 2, mitochondrial 

Protein Synonyms/Alias

 Dicarbonyl reductase HEP27; Protein D 

Gene Name

 DHRS2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
206	LALELAPKDIRVNCV	ubiquitination	[1]
219	CVVPGIIKTDFSKVF	ubiquitination	[1]
224	IIKTDFSKVFHGNES	ubiquitination	[1, 2, 3]
234	HGNESLWKNFKEHHQ	ubiquitination	[1, 2, 3]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2- propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21. 

Sequence Annotation

 NP_BIND 40 64 NAD or NADP (By similarity).
 ACT_SITE 185 185 Proton acceptor (By similarity).
 BINDING 172 172 Substrate (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleus; Oxidoreductase; Polymorphism; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 280 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005635; C:nuclear envelope; IDA:UniProtKB.
 GO:0004090; F:carbonyl reductase (NADPH) activity; IDA:UniProtKB.
 GO:0008207; P:C21-steroid hormone metabolic process; NAS:UniProtKB.
 GO:0034599; P:cellular response to oxidative stress; IDA:UniProtKB.
 GO:0043011; P:myeloid dendritic cell differentiation; IEP:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; NAS:UniProtKB.
 GO:0009636; P:response to toxic substance; IDA:UniProtKB. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR027052; DHRS2/4.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

  

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.