CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010096
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase 
Protein Synonyms/Alias
 Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase 
Gene Name
 spoT 
Gene Synonyms/Alias
 MPN_397; MP441 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
501NKLKKAVKYSELPLYacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity). 
Sequence Annotation
 DOMAIN 62 167 HD.  
Keyword
 Complete proteome; Hydrolase; Manganese; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 733 AA 
Protein Sequence
MFYNWLKLYK FSKMATLVEI ERDFLQKTAQ KFAPEVVALI TKALDYSKKW HGEQKRLSGE 60
PFFIHPLRTA LRLVEWNMDS NTVCAGLLHD IIEDTQVTEA DLTAIFGKEI TDLVVKVTKI 120
TSESKKQRQL NRKKEDLNLK SLVNIAMSSQ QEVNALVLKL ADRLDNISSI EFLAVEKQKI 180
IAKETLELYA KIAGRIGMYP VKTQLADLSF KVLDPKNFNN TLSKINQQKV FYDNEWGNFK 240
KQLEEMLEQN QIEYRLESRI KGIYSTYQKL TFHEQNIAKI HDLFAIRLIV KSELDCYHLL 300
GLIHLNFTVL MKHFKDYIAS PKQNFYQSIH TTVRLKGLNV EIQIRTQRMD HVSKYGFASH 360
WIYKEKKEGL LASALQVNYL NSKQMHSRDF FKRIFGTDII KVNVSSDNEP NIVKKLNVES 420
NSKLLDIAYE LYPKQFNKLE KIKLDGVEVM SFDVTAENEM VIEFCFGKTN NLKRRWLRYM 480
NNHVFRERVK KDLNKLKKAV KYSELPLYEK ALEELHLKLA DETQIKQRLN ALGIKKLTEF 540
LELIEYPHFP KNEHLYFLAS NNQKWRELIK PIKFALSQAV FQNSYFEQIE GIYITKIVIE 600
TCCTKIPDMP EQVIGILMKN ILRVHLHDCR ELANQKQPKI IPLYWNAHQL KMRPRKFRCQ 660
INIRGVWSET TVNKIVQTII EGDSYLERII PKIDKQKDEF ELNITMFIDN YHQLITIMEQ 720
ITTKNISYVW KYL 733 
Gene Ontology
 GO:0008893; F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0008081; F:phosphoric diester hydrolase activity; IEA:InterPro.
 GO:0015970; P:guanosine tetraphosphate biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR026020; (p)ppGpp_Synthase.
 IPR003607; HD/PDEase_dom.
 IPR006674; HD_domain.
 IPR004811; RelA/Spo_fam.
 IPR007685; RelA_SpoT. 
Pfam
 PF01966; HD
 PF04607; RelA_SpoT 
SMART
 SM00471; HDc
 SM00954; RelA_SpoT 
PROSITE
  
PRINTS