CPLM-009603

Genbank Nucleotide ID

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2

Protein Synonyms/Alias

G protein subunit beta-2; Transducin beta chain 2

Homo sapiens (Human)

Position	Peptide	Type	References
23	NQIRDARKACGDSTL	ubiquitination	[1, 2, 3, 4]
89	WDSYTTNKVHAIPLR	ubiquitination	[1, 5]
209	GACDASIKLWDVRDS	ubiquitination	[2, 4, 5, 6]
301	CNIWDAMKGDRAGVL	ubiquitination	[2, 4, 5, 6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]

Functional Description

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.

REPEAT 53 83 WD 1.
REPEAT 95 125 WD 2.
REPEAT 141 170 WD 3.
REPEAT 182 212 WD 4.
REPEAT 224 254 WD 5.
REPEAT 268 298 WD 6.
REPEAT 310 340 WD 7.
MOD_RES 2 2 N-acetylserine.
MOD_RES 239 239 Phosphotyrosine (By similarity).

Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat; Transducer; WD repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0044297; C:cell body; IEA:Compara.
GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0043234; C:protein complex; IEA:Compara.
GO:0005246; F:calcium channel regulator activity; IEA:Compara.
GO:0003924; F:GTPase activity; TAS:ProtInc.
GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
GO:0071377; P:cellular response to glucagon stimulus; TAS:Reactome.
GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
GO:0007186; P:G-protein coupled receptor signaling pathway; TAS:ProtInc.
GO:0007268; P:synaptic transmission; TAS:Reactome.

IPR020472; G-protein_beta_WD-40_rep.
IPR001632; Gprotein_B.
IPR016346; Guanine_nucleotide-bd_bsu.
IPR015943; WD40/YVTN_repeat-like_dom.
IPR001680; WD40_repeat.
IPR019775; WD40_repeat_CS.
IPR017986; WD40_repeat_dom.

PS00678; WD_REPEATS_1
PS50082; WD_REPEATS_2
PS50294; WD_REPEATS_REGION

PR00319; GPROTEINB.
PR00320; GPROTEINBRPT.