CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040685
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Probable E3 ubiquitin-protein ligase HECTD4 
Protein Synonyms/Alias
  
Gene Name
 HECTD4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
188SVGRGVSKLGSGLHGubiquitination[1]
2365PPPGQPAKGRVYFTYubiquitination[1]
2904YGLGHKVKRNGQLNLubiquitination[1]
3583TNVRPPKKVLEDQLTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Ligase; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4272 AA 
Protein Sequence
MGLLLVFPLI QSQSRTDPSL CNITAEVLLN CLRDCQPLSL TKEPADCLNG IETLLCSWLE 60
ETSDTGRHIP HKQKENAAAA LVALACARGS LKTFVHTVHL LQKQTDLGSL PVADVLYRLL 120
LLEGGPGSPS CLLGGKHIVS WGYEDMLPAP DSNTGSSSEN KDADLGRCLT ADGLYLYTTN 180
SVGRGVSKLG SGLHGTLRGF VYCRNEELEP GWVAFGSGSL LHRPVSFDNK PHSLFQVIDQ 240
NTLQVCQVVP MPANHLPIGS TMSTVHLSSD GTYFYWIWSP ASLNEKTPKG HSVFMDIFEL 300
VVENGVFVAN PLQERTILMR KEGESAKSIN EMLLSRLSRY RASPSATLAA LTGSTISNTL 360
KEDQAANTSC GLPLKMLRKT PIYTCGTYLV MLVPPPGGSG SSATRSLFGG TSGLSSLKIL 420
ASSLVYNISD GQFTSRADLI DAAGSSLGRG ALVPGLGACY DTVNNMLWTC SNDYIDQWCN 480
PGNQAFHYVC QRLGVSHIIT EPKEEAITTN EVINQLLHHV GAMCIHQLNL LATNPNLPIT 540
SVLGKQHPIE AHHLSSICDI MEKAMVNGDT CIIRCILVVF QVVFKFFFSP QTERNRDIIR 600
RSGLLLWQLL MAPKDQICPE IQKEVCLAIS SGLNILYPGE TEINNLLKLV LTEGERNSGL 660
SQLRDVILTN LAEQLQNNRF GSDEDDHYRL NDELLHYILK IVVRESCILI TKCQTVSKDD 720
FQKLLSTVPA ASSCLRYLMA VQNHLLSNTI LIKPDENDDS DSSLQGETLK ELKVSILALA 780
TQILTGCDEV LEMLQQVTTA LINSDIADRE QRLKGLEQVT KATMLGHLLP VLLTSLMHPN 840
LQTLIMADAL MPQLVQLVLY TSQTALLLKT QCPVFAEVGC SPCGAPDQKC RLFPDERMLE 900
EKEEPGFLTG LKIPAPWAAG KTVETVHPVR DNYKFKETVH IPGARCLYLR FDSRCSSQYD 960
YDKLVIYAGP NTNSRKVAEY GGNTLGYGSR SVLGTGWPKD LVKVEGDTVT FSFEMRSGRE 1020
HNTPDKAMWG FACTVRAQES SEDVSGGLPF LVDLALGLSV LACSMLRILY NGPEITKEEE 1080
ACQELLRSKL LQRCQWQVEA NGVISPALTP SPSPLPLTIE EDREFTYPSD VLVPPVGNYF 1140
DLPRIRLPPG IMIKLREISG RARPQFRPSI KEVIQPDVME EMVVSCVIKH LNLVDALQSL 1200
INFQYQEEHA EEYDLLCKIM GETFKKLNAM ERQLQSVAEL EQKWQSEVDD AMQGKLENNM 1260
PFFYDYHFNE NKMKELELLC SMKEVSFDGN DLENMVLSLR EKFLQEVNSL IQKPSHPLAK 1320
TKTLVKSLMN RAELLLHVTI AAQSGLTRSI SGTPAETPAC KSASETKVIS HAVRQPVFLR 1380
SMSAPSDLEM IGNEDLEFTR ANQRRRHVTS HRSSSFTLLQ SLAIEDSRDK PTYSVLLGQL 1440
FAFIGTNPDQ AVSSSSFLLA AQTRWRRGNT RKQALVHMRE LLTAAVRVGG VTHLVGPVTM 1500
VLQGGPRIEE LTCGGMVEQV QEAFGETMTS VVSLCARYPI ACANSIGLLC TIPYTRSEEK 1560
CLVRSGLVQL MDRLCSLSNQ TESSSSEKQT KKQKVATMAW AAFQVLANRC VEWEKEEGGS 1620
TEAVHSGLAR QVSSLLTNHL ARATECCGNQ AAGNDALQDV LSLLNDLSRS HIGKAILSQP 1680
ACVSKLLSLL LDQRPSPKLV LIILQLCRAA LPLMSVEDCG NVELPPWSYS VPSLNSEQED 1740
PSDPASKIAS LLLAKLADYV VPGCQTVLSP TASEPDTTLT KTSPKNSLKG DKDPGEESEA 1800
VDGKLSIFIH KREDQSSHEV LQPLLSSSEG RPFRLGTGAN MEKVVKMDRD MTKGGCCEVI 1860
TEEAAAALRK ATKWAQSGLI VSIGPPVESI NPETVSGLST GDKKKTAQTS ICRERNSELA 1920
RTDPVRPFIS GHVANSMAAE VIALLHSLLM APESNAAQIW TTTAEKVLSR ALMYIPQLGK 1980
YAESILENGS SSGRKLAKLQ RIARQAVAAL CALGGFKETI KIGSEVQVLG RGISGSIGVV 2040
ASINEQEGIA TVRFPPIDCR KTSQASDTLT IPLSRLCVPR SEALPLHKLS ITEKVVQAVQ 2100
SMLLPQEGSL SIHTSLPATG DGSAPVMAVV RLLAEIRTRA CLVMAQLLED SLFCEEFIQQ 2160
CPAAVEVLNL VAQECSAGER LAVVEVQCER LRMLYRDCAR PPPPPLQADR RQPKEITWSP 2220
SRVFPPVRAC MFSSHLTSVT FLADPSAGGG LPRGTFIYAT SPLPVQAPSF YWEIEIVSYG 2280
DTDDDTGPIV SFGFTTEAEK RDGAWTNPVG TCLFHNNGRA VHYNGSSLLQ WKSVRLDVTL 2340
SPGDVAGIGW ERTEGTPPPP GQPAKGRVYF TYCGQRLSPY LEDVSGGMWP VVHIQKKNTK 2400
TRANFGSRPF AYAEGQAHRN AADLCTDLAE EISANFEALP FAMASDSDND AGTSIASDPG 2460
THGPPCRIAA VATAQQQYDS DTSCHYKVEL SYENFITSGP DPHPPPIADD ESDDDDDDDI 2520
PQEDHYALLV KAWETKVFPT IRRRFRNEAE RKSGLDQIKG ALQLGMVDIA RQTVEFLYEE 2580
NGGIPRDLYL PTIEDIKDEA NKFTIDKVRK GLTVVTRSPD SNNVASSAVG TALPKFAIRG 2640
MLKTFGLHGV VLDVDSVNEL VQVETYLRSE GVLVRYWYPI DMLERPPAGY RRTATNGLVT 2700
LDNTNLQIHR ELLRCEAALA RLYCRMALLN IFAPKLPHLF TRLFHIPAIR DITLEHLQLL 2760
SNQLLAPPLP DGTISSSSIL LAQSLQHCIH SQNCSATDLF YQGNSQTVRE WLNVAITRTL 2820
HQGEESLLEL TKQICSFLQT APEQFPSEEF PISESKVNMD VNFPGAAFVV VSCKESQSGF 2880
RKDSSLYKAP WARVLVYGLG HKVKRNGQLN LIEAACYPRD ASPANTGLAP PPTADQYPSV 2940
VLSTDRVHIK LGVSPPPGAV LVLHSLPLEF PLAMAFAEQL LSWKSEDSEG KSEDEPDTIP 3000
TSVLLQVVEL LGNFLWTTDM AACVKELVFH LLAELLRTVH TLEQRRHPAG LSSSIALQLN 3060
PCLAMLMALQ SELHKLYDEE TQNWVSGGAC GGSGGAAAGD QGRFSTYFHA LMEGCLAVAE 3120
VTLPTNMSVT ASGVTSATAP NLSDSSSSSS SSPGQTPQSP SLLSKRKKVK MKREKASSSG 3180
KRQSSRTVDS DPTVLSIGGS KPEDMLWFHR ALTLLIILRH LTRKDPQGLG VTSDAIADAC 3240
QALVGPTAHS RLLVISGIPT HLDEGVVRGA IRKACNAHGG VFKDEIYIPL QEEDTKKPKD 3300
KAEGGDGKVE PEKTLAFPGT DSMEVSTSSS LTPAMSISAS ASTSQASICS SQGISQTVSD 3360
LSVDPLPAGL ELPIPPGLLE PHAVSSQESL DISLCSTGSL GSLGSLGEPL DNAETASVSD 3420
MGSMYTVTSL DNQPLAARPI KGFAVVEIRS RAKIEKIRAS LFNNNDLIGL SSLDGEDELM 3480
EMSTEEILTV SVVNQSLFDT QGSPGLEDYF NDKSIKGEKL VPGAREVLTE IFKSCAHSEQ 3540
TLSLTPAKPI RVSDIYLSKE QINSQTPGNL LHLFFTNVRP PKKVLEDQLT QILRKYGVPK 3600
PKFDKSKYSK AGKEQHPVKV VSTKRPITKP PAKDKAVLNS VSRTALSEKK PTVKPKSPEK 3660
SKPDEKDPEK SPTKKQEVPE EKYLTLEGFH KFVIDRARQD IRSVWRAILS CGYDLHFERC 3720
ACIDVRHAQK ASRKWTLEMD VALVQYINQL CRHLAITPAR LHPHEVYLDP ADAADPRVAC 3780
LLNVPIESLR LRFALLQSLN TTLETFFLPL VELRQTPMYT HSIAALLKEA KGLIFYDTKV 3840
TVMNRVLNAT VQRTADHAAP EITLDPLEIV GGEIRASENS YFCQAARQLA SVPSSQLCVK 3900
LASGGDPTYA FNIRFTGEEV HGTSGSFRHF LWQVCKELQS SSLSLLLLCP SSAVNKNKGK 3960
YILTPSPITY GEEQLLHFLG QLLGIAIRAD VPLPLDLLPS FWKTLVGEPL DPEQDLQEAD 4020
ILTYNYVKKF ESINDETELE ALCAEIASQH LATESPDSPN KPCCRFTYLT MTGEEVELCS 4080
RGRHILVAWE NKDIYAAAIR SLRLRELQNV ECVTAVRAGL GSIIPLQLLT MLSPLEMELR 4140
TCGLPYINLE FLKAHTMYQV GLMETDQHIE FFWGALEMFT QEELCKFIKF ACNQERIPFT 4200
CPCKDGGPDT AHVPPYPMKI APPDGTAGSP DSRYIRVETC MFMIKLPQYS SLEIMLEKLR 4260
CAIHYREDPL SG 4272 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:GOC. 
Interpro
 IPR000569; HECT. 
Pfam
 PF00632; HECT 
SMART
 SM00119; HECTc 
PROSITE
 PS50237; HECT 
PRINTS