CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012151
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lysosomal-associated transmembrane protein 5 
Protein Synonyms/Alias
 Lysosomal-associated multitransmembrane protein 5; Retinoic acid-inducible E3 protein 
Gene Name
 LAPTM5 
Gene Synonyms/Alias
 KIAA0085 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
217WRCYRLIKCMNSVEEubiquitination[1]
225CMNSVEEKRNSKMLQubiquitination[2]
229VEEKRNSKMLQKVVLubiquitination[3, 4]
233RNSKMLQKVVLPSYEubiquitination[1, 3, 4, 5, 6]
248EALSLPSKTPEGGPAubiquitination[1, 2, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 May have a special functional role during embryogenesis and in adult hematopoietic cells. 
Sequence Annotation
 MOD_RES 259 259 Phosphotyrosine (By similarity).  
Keyword
 Complete proteome; Lysosome; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 262 AA 
Protein Sequence
MDPRLSTVRQ TCCCFNVRIA TTALAIYHVI MSVLLFIEHS VEVAHGKASC KLSQMGYLRI 60
ADLISSFLLI TMLFIISLSL LIGVVKNREK YLLPFLSLQI MDYLLCLLTL LGSYIELPAY 120
LKLASRSRAS SSKFPLMTLQ LLDFCLSILT LCSSYMEVPT YLNFKSMNHM NYLPSQEDMP 180
HNQFIKMMII FSIAFITVLI FKVYMFKCVW RCYRLIKCMN SVEEKRNSKM LQKVVLPSYE 240
EALSLPSKTP EGGPAPPPYS EV 262 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; TAS:ProtInc.
 GO:0006810; P:transport; IEA:UniProtKB-KW. 
Interpro
 IPR004687; LAPTM4/5.
 IPR018396; LAPTM_4A/5.
 IPR018397; Lysosomal-assoc_TM_prot5. 
Pfam
 PF03821; Mtp 
SMART
  
PROSITE
  
PRINTS